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Questions related to Quantitative RT-PCR
Hello! Has it happened to you that the fetal bovine serum is contaminating and eventually (not immediately) your cells become contaminated? If so, what did you do to fix the problem? Thank you!!!
23 March 2023 2,031 5 View
I'm a PhD student working on protein engineering & immunology and making recombinant proteins in lab for work with mammalian systems. I have tried using ClearColi previously (BL21 DE3 w/...
22 March 2023 5,698 3 View
I need to compare data of two ratios. The ratio obtained by treatment : control reading from qPCR. I am not sure whether the conventional t test would be ok to determine significance between two...
16 March 2023 1,545 2 View
I have a set of data from three groups across 3 time points (2 groups are subjected to an intervention and the third acts as control). I want to evaluate intra and inter-group differences between...
09 March 2023 6,704 3 View
Anyone knows name of a journal for cancer research where I can publish freee of cost or low cost.
07 March 2023 3,664 2 View
Guillermo Zalba is not part of the team of the article, plese delete his name Best regards Alejandra Guillermina Miranda-Díaz, MD, PhD
02 March 2023 7,229 2 View
I'm a little confused, I am doing a PCR reaction that requires 100ng of genomic DNA template, and the concentration of my genomic DNA is 591,9ng/ul. So is 100ng of the template the same as saying...
26 February 2023 9,408 3 View
What factors affect melt peak [-d(RFU)/dT) in real-time PCR? What should I do if I want to increase my melt peak [-d(RFU)/dT)?
23 February 2023 160 2 View
I've done a qPCR reaction using Bio-Rad CFX Duet, 10 ul reaction, using EvaGreen dye. as you can see, image 01 is what I think as a good amplification plot. Today I do the qPCR again using a...
17 February 2023 4,196 3 View
I have four groups, the anova accepted the hypothesis of equal means. But when I used the two sample t-test 6 times (4 combination 2), two of these 6 tests are significant, which method we prefer?
16 February 2023 6,937 1 View
What is main difference between Ct and Cq value? When do we use Ct value and when Cq In Real Time PCR? How to determine ct/cq value in a given qPCR assay?
15 February 2023 5,170 5 View
I did prepare 1litre of comet assay lysis solution by adding sodium lauryl sarcosinate. For the assay used only 100 or 150ml and kept the remaining at 4 degree celsius for future use. After 2...
14 February 2023 2,312 4 View
As per the method described by Aebi (1984), extinction coefficient for catalase is 39.4 L/micromoles/cm. but in many research articles, the given extinction coefficient is 39.4 L/mM/cm and 40...
12 February 2023 1,165 1 View
Any optimized protocol for gene knockdown in THP1 cells using siRNA?
09 February 2023 6,528 0 View
I performed an immunohistochemical study and I have estimated the percentage of expression of different tumor markers, in this case, can I estimate fold changes with respect to the control ?
08 February 2023 3,600 6 View
Our main problem is that organoids embedded in agarose cannot be traced. Thank you!
29 January 2023 2,056 1 View
Hello I wish good health to all my friends. I had two questions. 1: Is free vitamin D in serum the same as vitamin D in VDBP in terms of immunological properties and can it be identified with a...
21 January 2023 3,970 1 View
In analyzing data from my qPCR experiments, analysis on GraphPad is done using the ddCt, in presenting the analysis in graph, which is the most appropriate, the relative fold change or the ddCt?
06 January 2023 9,042 0 View
I used a KOD DNA polymerase for PCR that works very well. Then I used this enzyme to run a TaqMan probe real-time PCR but the results waere unexpected. I attached the results. The fluorescence...
31 December 2022 6,464 4 View
With good pictures?
27 December 2022 3,720 3 View
analysis of lipids in GC/MS
26 December 2022 152 1 View
Our oligonucleotides anneald stick-to-stick tail during PCR. Why another possible variants were not presented (or presented in very small amount)?
05 December 2022 6,521 1 View
Hi all, I am looking to test relative gene expression for my gene of interest in comparison to a reference gene. I realised, belatedly, I'd need a standard curve for every plate and I am wondering...
03 December 2022 1,453 0 View
Hi all, my name is David Previously I cloned a protein and everything was perfect. However now I want to clone this same protein but with a PelB sequence in its N-terminal for that I designed the...
02 December 2022 5,562 1 View