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Questions related to Quantitative RT-PCR
Comprehensive Overview of Myocardial Infarction: Etiology, E... What is the role of primary percutaneous coronary intervention (PCI) in the management of STEMI?
16 June 2024 2,307 1 View
What's your suggestion for converting a heavy organic alcohol to its corresponding acid (via oxidation or dehydrogenation)? Your industrial experience is appreciated.
13 June 2024 3,927 3 View
How mat I export in a Tab-delimited (win) file?
11 June 2024 7,726 1 View
Hi there, I have transfected these two plasmids into 293T packaging cells to encapsulate a pLVX-M-Puro coding plasmid. I had already validated the stability of the pLVX-M-Puro insert by transient...
07 June 2024 8,401 5 View
Products / Services Groceries Fancy items Garments Gifts Electronic Goods and Accessories Household Supplies Skin Care Products Fitness or Wellness Products Jewellery Hotel...
21 May 2024 6,436 0 View
Can you explain the pathophysiology underlying SIADH and DI Advances in Understanding and Management of Antidiuretic Hor...
09 May 2024 5,480 2 View
I am trying to use Mallows Cp to select best NIR calibration model. For each sample-spectrum there are 700 wavelengths (high degree of multicollinearity), so, there are potentially 700 predictors....
29 April 2024 3,072 0 View
How can we evaluate changes/impacts of activities within one research period?
19 April 2024 9,072 1 View
Point of view La relevancia y actualidad de esta problemática en el campo de la salud mental radican en la necesidad creciente de proporcionar atención de calidad, accesible y personalizada a un...
15 April 2024 8,106 0 View
I want to add a doi to some of my texts.
14 April 2024 3,848 1 View
There is a hierarchical structure in academic leadership which often favours the ones with most successful grants or fame in their own field. But leadership is a different ball game. Should...
06 April 2024 7,110 7 View
Cattle egret eats insects on mango flowers, which ecological interaction would we consider this an example of?
31 March 2024 5,460 0 View
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30 March 2024 3,488 3 View
I attempted a quick test to generate the .gro file from the .pdb file, which I initially copied from our main source. I obtained this error: "Atom OA in residue MET 1 was not found in rtp entry...
18 March 2024 9,004 0 View
I'd like to know the type of AAV and promoter and the best effective time on the rat skin. Any advice on the injection method would also be helpful. Thanks.
11 March 2024 9,779 1 View
Im looking to use H2O2 with my HEK293 cells to cause a stress response in order to see if the cells become more sensitive to externally applied electromagnet fields. I need the cells to still be...
06 March 2024 8,520 1 View
Ran out of RT in Illumina mRNA prep Kit, unfortunately the company doesn't sell it separately; plan to substitute it with Superscript III. Does anyone done that before? Will it work?
21 February 2024 1,325 0 View
Hello all I'm working with SHSY5Y cells and I always made Cryo cells from them, them thawed them and used them without problem. But, recently, after thawing, they are remain floating and not...
20 February 2024 9,350 2 View
I have performed RNA extraction of blood sample using TRIzol method. I ran RNA samples on non-denaturing gel (1.5%) that showed extreme DNA contamination and degraded RNA band. Please suggest me...
22 January 2024 2,574 4 View
Dear all, I recently had trouble analyzing my data by Western blot. I'm using the cell line model and transfection performance to analyze the importance of my target protein to cellular...
19 January 2024 6,251 2 View
I am docking multiple ligands (new designed ligand) against my protein using Autodock Vina in Chimera. The results displayed in this program are somewhat strange, because in some cases the ligands...
17 January 2024 9,640 3 View
I subcutaneously performed implantation in rats and harvested the sample after 1, 2, 3, and 6 months. The implant is decellularized human skin. The procedure of how my lab did it was drawn in the...
16 January 2024 4,174 3 View
I have a protein embedded in lipid bilayer. At some point, my protein moved towards the edge of simulation box. I applied PBC wrapping so the box is centered around protein. However, the...
30 December 2023 2,072 0 View
I'm wondering if lysozyme could also affect nucleases like RNase. My understanding is that lysozyme hydrolyses glycosidic bonds in bacterial cell walls, specifically between N-acetylmuramic acid...
07 December 2023 2,824 2 View