Our oligonucleotides anneald stick-to-stick tail during PCR. Why another possible variants were not presented (or presented in very small amount)?
because I like this.
sodium ion battery is not taking higher current when charging and also has a high voltage drop
06 May 2024 3,182 1 View
I need a research topic related to recent trends in GST
16 April 2024 1,805 0 View
I am trying to predict the stability of a protein with different SNP. I tried using DUET, Predict SNP and Dynamut. The problem with DUET is that I cannot do double mutation however, it gives fast...
29 February 2024 2,758 3 View
I am currently learning about PyMol to utilize in my project. I used PyMol to visualize potential H-bond interactions in specific amino acid residues. However, I have discovered that Arg465 and...
16 February 2024 7,922 3 View
Dear colleagues, I am currently in a search of Q1-Q2 journal that is specialized on general mycology and is free of publication or any other charge. I would like to publish manuscripts on ecology...
23 January 2024 6,749 4 View
Dear collegues! Is there free database with list of common (typical) inorganic chemistry reactions, which widely spread across school and student books? Like Reaxys, Scifinder but with an eye...
15 January 2024 232 4 View
Looking to simulate a 3-tier heterogeneous network. The base stations in each tier operate in a different frequency band, have a different transmit power and path loss exponent. Some have sector...
20 November 2023 1,053 6 View
Thank you this question has been answered.
02 November 2023 7,140 10 View
I am seeking guidance on how to integrate a VANET file, generated using SUMO, which encompasses the road network, vehicles, and routes, with NetSim. Specifically: What steps are essential for...
04 October 2023 6,652 3 View
The Tm of primers is 65 degrees and I am getting only non specific bands around 1kb. I amplified using Q5-HF Polymerase. How can I improve the specificity to get my desired band?
17 September 2023 4,956 5 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...
06 August 2024 3,130 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
A Markov-like Model for Patient Progression" Markov Chain Monte Carlo (MCMC) Markov Chain Monte Carlo (MCMC) is a powerful computational technique used to draw samples from a probability...
05 August 2024 10,079 0 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
Read the journal article by Douglas M. Lambert, “The Eight Essential Supply Chain Management Processes,” Supply Chain Management Review, Vol. 8, No. 6 (2004), pp. 18-26
04 August 2024 9,919 4 View
How does the atmosphere affect the flow of matter and energy on Earth and flow of energy in the biosphere related to the flow of food through a food chain?
02 August 2024 9,644 0 View