Our oligonucleotides anneald stick-to-stick tail during PCR. Why another possible variants were not presented (or presented in very small amount)?
because I like this.
I have a genotype gvcf of significant size, which is causing an issue in analysis. I was wondering if there is a way to get around the size issue and run the genotype gvcf in R or do I need to...
09 February 2021 4,178 4 View
I am working on SOC estimation of battery for EV's, I want to extract the real battery data through the experiments on battery, so i want to know what exactly the equipment's i need to perform the...
22 January 2021 8,216 2 View
What if in column chromatography with solution (neutral pH) acidic pH is observed and remains along the whole process? What went wrong and what to do with this?
10 April 2020 5,587 4 View
I'm currently working on a research of thermal-hydraulic performance of artificially roughened pipes using Ansys Fluent. I have a 2D model of pipe section (upper boundary is wall with rectangular...
24 March 2020 623 3 View
For example i use Leica RTC360 scanner for terrestrial data acquisition and i like to perform a stitching with point cloud gathered from UAV photos (not lidar). What is the best way to do so? I am...
03 March 2020 9,471 3 View
I am getting a background dye reading at 473nm. Does this dye give a reading only under UV or is getting this background reading normal?
24 February 2020 9,426 1 View
Sodium hypochloride and Ethanol have been used for surface sterilisation. May I know any alternate methods for surface sterilisation?...
05 January 2020 9,237 2 View
I am an undergraduate student currently working on a group mini-dissertation. (forgive me for the rudimentary knowledge I have) We're studying the co-relation between peoples' attitudes toward the...
27 August 2019 7,734 2 View
Hello, I have started my research work in the area of cognitive radio networks. Initially I am looking to design a CRN environment in Matlab. Please help me with the initial requirements required...
06 February 2019 2,145 3 View
Dear colleagues, I am trying to search for boron-aluminum clusters global minima using GMIN code developed by Wales group. Could anyone help me please with the input file? If there are...
29 October 2018 6,686 1 View
Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...
02 March 2021 4,029 3 View
Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....
02 March 2021 285 2 View
So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...
02 March 2021 1,146 2 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View
To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...
01 March 2021 8,683 4 View
Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?
01 March 2021 5,311 1 View
Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.
28 February 2021 5,008 4 View
I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...
27 February 2021 547 3 View
Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...
26 February 2021 4,777 4 View
Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...
26 February 2021 5,029 2 View