Contact experts in Quantitative RT-PCR to get answers
1,530 views 1,226 posts
Questions related to Quantitative RT-PCR
I am wondering which are the antibody commercially available to detect specifically the proteasome subunits in budding yeast (Saccharomyces cerevisiae). Any suggestions and recommendations really...
18 July 2023 5,165 0 View
Hello, I have a small sample size n=3, in each animal, I am analysing 2 slices. This is due to the time limit with the facility. Midway, I had to also use a different microscope but I tried to...
16 July 2023 4,438 3 View
My workflow involves creating fluorescently barcoded regions on a spleen sample. I then dissociate the sample in saline solution and take it to the flow core for sorting of the positive vs...
09 July 2023 7,012 3 View
What is the step to step mechanism/process for thin film deposition in the Sputtering process?
09 July 2023 6,550 5 View
I am supposed to be doing an NEM Alkylation treatment on mouse pups at e13 (which have been electroporated with plasmid) before perfusion with PFA. I wasn't sure what the purpose was, and what...
07 July 2023 7,775 1 View
If I treat dH20 with 0.1% (v/v) DEPC and autoclave it, is the water still RNase free and can I use it as RNase-free water? Or is it ONLY ddH20 with DEPC and NOT autoclaved that can be used as...
06 July 2023 6,445 3 View
Can anyone recommend the working concentration of DAPI for staining pollen cells
04 July 2023 2,318 2 View
Hello I need to compare my “control” group with all other groups that received a dietary supplement with three different ratios. Now I want to compare my control group with the other three groups...
29 June 2023 5,926 1 View
If nanodrop facility is not available, can we predict the dna conc. from the electrophoresis picture?
21 June 2023 9,266 3 View
Let's say that I want to check the associations of mild-exercise to hypertension. I have a set of variables (apart from the IV Of interest and DV): a. sex b. race c. smoking status In my...
21 June 2023 626 0 View
How do you store and preserve RNA samples to maintain their integrity and stability over time, and what are some of the best practices for long-term storage?
17 June 2023 8,515 3 View
??
17 June 2023 1,519 1 View
So I learned that after an ANOVA of that kind you want to do a post hoc test, which in this case of 3 levels could be either Tukey or a recoding into dummy variables of 2 of the 3 levels. I am new...
15 June 2023 8,833 0 View
I have a word file and want to sent via an email but I am unable to compress it without changing the format. I need it to be in word as it has track changes responses.
13 June 2023 8,405 3 View
Antihypertensive Medications - StatPearls - NCBI Bookshelf (nih.gov)
08 June 2023 212 3 View
hypertension drug
05 June 2023 6,786 9 View
recently I bought the Fisher brand pipette tip:...
30 May 2023 6,103 2 View
done
24 May 2023 9,239 1 View
Hello, Does anyone know, have any tips on (the settings) to view Immunohistochemistry mice brain slices for anti -rabbit TFeb and Notch w/ DAB staining? Any insights on which settings you use...
24 May 2023 8,668 0 View
Hello Everyone, I want an exosome mediated delivery of mRNA, for that exosome loading is required through electroporation. I searched a lot for an optimized protocol for electroporation of mRNA...
21 May 2023 3,771 1 View
Hello, I'm planning to sample live snails and ship them internationally. My goal is to immerse live snails in liquid nitrogen to reduce expression noise, then grind the tissues and use a...
12 May 2023 6,804 2 View
Hello all, I am preparing a diagnostic kit for the detection of viral nucleic acid and testing them using Taqman probes. I am getting initial rise of the curves by around 1000 RFU and then...
11 May 2023 4,740 2 View
When I record the basal activity of isolated heart perfusion in mice, I see rhythmic changes in the intensity of the left ventricular contraction. I mean left ventricular systolic and developed...
11 May 2023 9,642 0 View
Hello and good day all, I have encountered issues with my RNA extraction using the Trizol method. I have attempted to optimize the protocol for both yield and purity, but the results have not met...
09 May 2023 2,528 1 View