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Questions related to Quantitative RT-PCR
How a well structured agricultural marketing system helps farmers to increase their income and importance of market structure on producers and consumers?
27 June 2024 2,304 3 View
Hello, I have been performing Western Blots to study the protein levels of a rodent skin and muscle sample. However, I have not been able to produce homogenous reference bands. I suspect that...
27 June 2024 5,952 3 View
I recently isolated mice cardiomyocytes and found some are not individual cells. I tried to stain the cells with Wheat Germ Agulutinin, it did not work. Does anyone have recommendations on the...
21 June 2024 3,257 2 View
We utilize an immortal endothelial cell line and various agonists to observe their effects on Src phosphorylation. However, our protocols seem to induce high basal levels of Src phosphorylation....
18 June 2024 1,673 0 View
Comprehensive Overview of Myocardial Infarction: Etiology, E... How are cardiac troponins used in the diagnosis of myocardial infarction?
16 June 2024 7,124 1 View
Comprehensive Overview of Myocardial Infarction: Etiology, E... What is the role of primary percutaneous coronary intervention (PCI) in the management of STEMI?
16 June 2024 2,290 1 View
What's your suggestion for converting a heavy organic alcohol to its corresponding acid (via oxidation or dehydrogenation)? Your industrial experience is appreciated.
13 June 2024 3,895 3 View
Hi there, I have transfected these two plasmids into 293T packaging cells to encapsulate a pLVX-M-Puro coding plasmid. I had already validated the stability of the pLVX-M-Puro insert by transient...
07 June 2024 8,310 5 View
Products / Services Groceries Fancy items Garments Gifts Electronic Goods and Accessories Household Supplies Skin Care Products Fitness or Wellness Products Jewellery Hotel...
21 May 2024 6,410 0 View
Can you explain the pathophysiology underlying SIADH and DI Advances in Understanding and Management of Antidiuretic Hor...
09 May 2024 5,458 2 View
I am trying to use Mallows Cp to select best NIR calibration model. For each sample-spectrum there are 700 wavelengths (high degree of multicollinearity), so, there are potentially 700 predictors....
29 April 2024 3,054 0 View
How can we evaluate changes/impacts of activities within one research period?
19 April 2024 9,060 1 View
I want to add a doi to some of my texts.
14 April 2024 3,834 1 View
There is a hierarchical structure in academic leadership which often favours the ones with most successful grants or fame in their own field. But leadership is a different ball game. Should...
06 April 2024 7,091 7 View
Cattle egret eats insects on mango flowers, which ecological interaction would we consider this an example of?
31 March 2024 5,430 0 View
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30 March 2024 3,472 3 View
I attempted a quick test to generate the .gro file from the .pdb file, which I initially copied from our main source. I obtained this error: "Atom OA in residue MET 1 was not found in rtp entry...
18 March 2024 8,990 0 View
I'd like to know the type of AAV and promoter and the best effective time on the rat skin. Any advice on the injection method would also be helpful. Thanks.
11 March 2024 9,764 1 View
Im looking to use H2O2 with my HEK293 cells to cause a stress response in order to see if the cells become more sensitive to externally applied electromagnet fields. I need the cells to still be...
06 March 2024 8,505 1 View
Ran out of RT in Illumina mRNA prep Kit, unfortunately the company doesn't sell it separately; plan to substitute it with Superscript III. Does anyone done that before? Will it work?
21 February 2024 1,311 0 View
Hello all I'm working with SHSY5Y cells and I always made Cryo cells from them, them thawed them and used them without problem. But, recently, after thawing, they are remain floating and not...
20 February 2024 9,326 2 View
I have performed RNA extraction of blood sample using TRIzol method. I ran RNA samples on non-denaturing gel (1.5%) that showed extreme DNA contamination and degraded RNA band. Please suggest me...
22 January 2024 2,558 4 View
Dear all, I recently had trouble analyzing my data by Western blot. I'm using the cell line model and transfection performance to analyze the importance of my target protein to cellular...
19 January 2024 6,240 2 View
I am docking multiple ligands (new designed ligand) against my protein using Autodock Vina in Chimera. The results displayed in this program are somewhat strange, because in some cases the ligands...
17 January 2024 9,624 3 View