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Questions related to Quantitative RT-PCR
I am currently conducting a literature review on the effects of tactical games (e.g., team sports, small-sided games, or strategy-based activities) on executive function. Specifically, I am...
last month 3,242 0 View
Dear collegues Have you evetr tried to estimate the level of the soluble form of IL-17RA in himan serum? We used ELISA Kit IL-17RA "FineTest" (China) for the analysis and have found a division of...
24 July 2025 9,608 1 View
Esta pregunta surge de una investigación filosófica que articula los conceptos de Autosimilitud, Trascendencia Humana y pluriversalidad en el marco de epistemologías transdisciplinarias aplicadas...
19 July 2025 8,616 0 View
I’m working with EC-Lab v11.61 to analyze my supercapacitor data. When I attempt to export the data as a .txt file using the Text File Export option, I encounter an issue where the status at the...
17 May 2025 5,430 0 View
I am a Year 4 student now working for my final year project. My topic is using cerium oxide nanoparticles system to remove the sul1 gene in RP4 E. coli. In my preliminary study, we discovered that...
15 March 2025 8,568 5 View
I dont know how much information i will need to provide, so i will give it a go: Im trying to characterise a GFP expression vector response on acetic acid overflow metabolism, therefore i...
14 March 2025 8,525 5 View
I'm trying to do an endothelial tube formation assay, but I'm having trouble with Corning's Matrigel used for this. The CoA states that the protein concentration is 9 mg/mL. But in the manual of...
25 February 2025 4,435 1 View
Estoy realizando una investigación-intervención con terapia narrativa de corte cualitativo con una familia multiproblemática migrante, y tomando en cuenta a Wainstein y Wittner (2017), quienes...
21 January 2025 1,023 2 View
When I transfected macrophages with Thermo fisher MessengerMAX, I found that the mRNA transfection efficiency was about 20%-30%. Is there any way to improve the mRNA transfection efficiency or...
13 December 2024 1,322 3 View
Hi everyone, I'm working with a soluble recombinant protein that contains a SUMO tag, which facilitates the downstream purification process. However, when I tried to purify the protein using...
12 November 2024 2,941 6 View
# (c) Silvaco Inc., 2015 go atlas set width=500 mesh width=$width x.mesh location=-2.5 spacing=0.5 x.mesh location=-1.2 spacing=0.1 x.mesh location=-1 spacing=0.1 x.mesh location=0...
04 November 2024 8,617 0 View
E.g. what compound is more stable SmF3 or Sm2O3? Based on e.g. HSC software calculation one can get delta H (of formation) for both compounds and the lower value (more negative) indicates the more...
04 November 2024 1,180 0 View
Many women think of masturbation as unnatural and disgusting and a complete waste of time, and don’t understand why anybody does it and are unsympathetic to the view that people might continue to...
30 October 2024 8,080 2 View
Erotic stimulation … effects a series of physiologic changes which … appear to involve adrenal secretion; … increased pulse rate; … a flow of blood into … the penis … and the clitoris; … often...
26 October 2024 8,322 6 View
Do you place the Directed Repeat fixed sequence on the 5' end or 3' end of your spacer sequence when designing Cas13 crRNAs? Does it matter? Thank you
25 October 2024 1,197 2 View
I want field guide for collection of dragonflies and damselflies to study taxonomy of odonates
10 October 2024 4,161 1 View
Every wish, every fear and terror, every fragment of the past and present must be shared with each other, as if only in this way can the relationship between husband and wife become closer. But...
05 October 2024 8,729 0 View
Hello everyone, I am attempting to culture HUVECs in a collagen and laminin hydrogel. The cells are mixed with the polymer solution, which is then allowed to gel. However, I have noticed that the...
19 July 2024 9,848 2 View
I included the article sent to me to Saved Articles List but do not know how to get it.
14 July 2024 308 1 View
How a well structured agricultural marketing system helps farmers to increase their income and importance of market structure on producers and consumers?
27 June 2024 2,321 3 View
Hello, I have been performing Western Blots to study the protein levels of a rodent skin and muscle sample. However, I have not been able to produce homogenous reference bands. I suspect that...
27 June 2024 6,020 3 View
I recently isolated mice cardiomyocytes and found some are not individual cells. I tried to stain the cells with Wheat Germ Agulutinin, it did not work. Does anyone have recommendations on the...
21 June 2024 3,301 2 View
We utilize an immortal endothelial cell line and various agonists to observe their effects on Src phosphorylation. However, our protocols seem to induce high basal levels of Src phosphorylation....
18 June 2024 1,698 0 View
Comprehensive Overview of Myocardial Infarction: Etiology, E... How are cardiac troponins used in the diagnosis of myocardial infarction?
16 June 2024 7,155 1 View