Contact experts in Quantitative RT-PCR to get answers
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Questions related to Quantitative RT-PCR
I am facing a problem getting good quality RNA; if anybody suggests me, It would be great.
09 August 2023 5,115 2 View
A quantitative RT PCR assay was performed with a panel of low, weak, medium and high positive and negative members in one batch for 5 days at 3 different (sites, machines and operator). How to...
07 August 2023 9,062 0 View
My workflow involves creating fluorescently barcoded regions on a spleen sample. I then dissociate the sample in saline solution and take it to the flow core for sorting of the positive vs...
09 July 2023 6,985 3 View
What is the step to step mechanism/process for thin film deposition in the Sputtering process?
09 July 2023 6,528 5 View
If I treat dH20 with 0.1% (v/v) DEPC and autoclave it, is the water still RNase free and can I use it as RNase-free water? Or is it ONLY ddH20 with DEPC and NOT autoclaved that can be used as...
06 July 2023 6,422 3 View
Can anyone recommend the working concentration of DAPI for staining pollen cells
04 July 2023 2,292 2 View
How do you store and preserve RNA samples to maintain their integrity and stability over time, and what are some of the best practices for long-term storage?
17 June 2023 8,497 3 View
I have a word file and want to sent via an email but I am unable to compress it without changing the format. I need it to be in word as it has track changes responses.
13 June 2023 8,383 3 View
hypertension drug
05 June 2023 6,771 9 View
done
24 May 2023 9,221 1 View
Hello Everyone, I want an exosome mediated delivery of mRNA, for that exosome loading is required through electroporation. I searched a lot for an optimized protocol for electroporation of mRNA...
21 May 2023 3,755 1 View
Hello, I'm planning to sample live snails and ship them internationally. My goal is to immerse live snails in liquid nitrogen to reduce expression noise, then grind the tissues and use a...
12 May 2023 6,786 2 View
Hello all, I am preparing a diagnostic kit for the detection of viral nucleic acid and testing them using Taqman probes. I am getting initial rise of the curves by around 1000 RFU and then...
11 May 2023 4,721 2 View
When I record the basal activity of isolated heart perfusion in mice, I see rhythmic changes in the intensity of the left ventricular contraction. I mean left ventricular systolic and developed...
11 May 2023 9,622 0 View
Hello and good day all, I have encountered issues with my RNA extraction using the Trizol method. I have attempted to optimize the protocol for both yield and purity, but the results have not met...
09 May 2023 2,512 1 View
Howdy all! I wanted to enquire as to the possible cause of magnetic bead contamination in final sample elution’s while using the ZymoBiomics MagBead RNA kit. We are doing many extractions in...
09 May 2023 4,654 0 View
Currently I am doing RNA immunoprecipitation to find the targets of an RNA binding protein. I am unclear about the reverse-crosslinking protocol. I have found two approaches one in which they wash...
04 May 2023 9,740 2 View
We performed several PCR assays to detect virulence factor of pathogenic strains, but a faint band amplified on negative control. After this, we have changed all reaction components (one by one)...
27 April 2023 6,605 3 View
If it's the case, what were the proliferation and cell growth status before you realized that it was due to a Mycoplasma infection? Thank you in advance.
12 April 2023 8,808 0 View
I've performed a colony PCR with two unknown bacteria (in triplo). Lanes 6, 7 and 8 shows one bacteria with a nice outcome. The band that I expect is 1465 bp, because during the colony PCR, I use...
06 April 2023 7,046 10 View
cell culture: HEK293F medium: BalanCD, without antibiotics
31 March 2023 8,822 1 View
I'm doing PCR and qPCR frequently. My assay is extremely sensitive so I need to pipette a very exact volume from my SOP. From my experience and also my school lab course, I learned to press the...
30 March 2023 961 4 View
When we do a nucleotide blast for certain sequences, there are tons of results like "cloning vector" or "synthetic construct". These are plasmids made by researchers. I want to exclude all these...
26 March 2023 4,520 3 View
Does DMSO can have cytotoxic effects on microorganisms and may interfere with the results of microbiological assays? What is the concentration that is used to secerning the antimicrobial activity...
23 March 2023 6,654 3 View