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Questions related to Quantitative RT-PCR
What is the unique anti-aging property of Glutathione (GSH)? I think this molecule is not the only one that works great! In theory, many molecules can be created for the great anti-aging property!...
26 December 2021 6,920 7 View
Has anyone successfully blotted both liver and muscle actin in chicken with a single antibody on a Western? We have purchased two antibodies for actin from MBL and from Santa Cruz. The MBL...
16 November 2021 9,039 0 View
Hi Do you know/recommend an ideal substitute for the immersion oil for fluorescence microscopy, the Immersol 518F from Zeiss? Thank you!
11 November 2021 6,810 0 View
I performed WB with 15 ug/10 ul protein sample for 1 lane My expected band is indicated in red marker in the image. But something wrong with my band, always. Band is connected by strong dots...
25 September 2021 8,596 0 View
I am studying photocatalysis of Polystyrene nanoplastics (22 nm) with TiO2 18 nm (%80 Anatase, %20 Rutile) under UVC in DI water (15 mL solution in 20 mL vials. Concentrations: 20 ppm PS, 20 ppm...
18 September 2021 7,289 5 View
I have very few copper mesh to isolate my InVivo ephy implants and I'm trying to find a distributor in Europe but still not successful found one that sells small amount of it. The item reference...
14 September 2021 3,966 0 View
I am imaging cells which have endogenous GFP signal and I stained them with DAPI in mounting medium. I use confocal ZEISS LSM800. Some of the cell soma (looks like cytoplasm and processes) seems...
14 September 2021 9,414 4 View
I am looking for a lock box to store a biotoxin in my -80 freezer? Does anyone know of any that can withstand temperatures that low? Thanks}!
03 September 2021 3,401 0 View
I am experimenting degradation of polystyrene nanoparticle using TiO2 and I face higher final TOC values than initial TOC. I assume it is due to the generation of CO2 while mineralization. However...
15 August 2021 4,203 5 View
Hello everyone, I have conducted a multi-phenotype GWAS analysis. I have 5 phenotypes in total, the GWAS lambda ranged from 0.8 to 1.12, only two of them are acceptable (0.985, 1.030). Is there...
10 August 2021 6,355 0 View
Hi, I wish to do automatic orca calculations by putting input files in a queue. For example, I have done geometry optimization, using this optimized structure, I wish to go for Frequency, TDDFT...
08 August 2021 8,242 1 View
I am having trouble with Nanog IHC in ESCs and iPSCs. In all of the cell lines that I am characterizing, I have measured mRNA expression of Oct4, Sox2, and Nanog, and I have successfully stained...
19 July 2021 3,534 1 View
I have cDNA samples extracted from 90+ individuals which I intend to utilise in a series of qPCR experiments to assess the influence of a specific SNP genotype/s on target gene/s expression. I...
09 July 2021 3,025 3 View
Can I use 'quantitect reverse transcription kit' storaged in refrigeration temperature for 20 days? Originally, it should be stored at -20 celsius temperature scale.
22 June 2021 6,950 3 View
I want to isolate extracellular DNA from 1L of environmental sample (lake water). Is it possible to concentrate the ext. DNA in sample using anion exchange columns ? What are important...
21 June 2021 7,683 0 View
Hi everyone. For my project I have to reduce the time for reverse transcription of mRNA. We are using High Capacity cDNA Reverse Transcription Kit from Applied Biosystems. It's recommended there...
16 June 2021 8,308 3 View
Hi folks I published a couple of things in Nature Education Knowledge and they were well received; but they stopped producing new content. Is anyone aware of similar educational outlets? I'm...
08 June 2021 1,551 0 View
Explain the unequal control and treatment group effect on choices of econometric methods
08 June 2021 6,451 5 View
Good day great scholars. Please I would appreciate help on how to install Discovery Studio and PyRx in Ubuntu. I will also like to know how I can assess/identify the path of the License Pack for...
27 May 2021 9,840 10 View
Myocardial infarction usually affect heart function. I wish to know if this pathological condition can affect the level of gastric acid production in the body and hence the digestive process.
26 May 2021 5,746 6 View
We are struggling with inconsistent settlement and early life survival with sea cucumber (sandfish, Holothuria scabra) in a semi commercial hatchery in tropical Northern Territory, Australia. We...
19 May 2021 3,360 0 View
I try to develop a gradient hplc analysis method for two drugs/ the 2nd one composed of 3 compounds, but an unknown peak appears at 8.5 min as shown in the attached photo of injected blank...
12 May 2021 4,065 3 View
Does anyone have experience growing single cell clones of MCF-10A.I have generated gene knockouts and need to pick single cell clones from these cell lines. It is challenging as these cells are...
29 April 2021 1,895 2 View
I am doing a Chip-seq experiment on one human sample and testing the DNA segment I got from Chip by qPCR first. What I found is my primer designed based on UCSC Genome Browser doesn't work very...
19 April 2021 2,447 2 View