Contact experts in Quantitative RT-PCR to get answers
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Questions related to Quantitative RT-PCR
I have been trying to find the copy number variation for the knocked in mCherry gene in U2OS cells. I am using the Thermofisher TaqMan Copy Number Assay kit with the custom probe for the mCherry...
12 April 2021 1,863 0 View
Hello friends! I have coded VUMAT subroutine and when i submitted the job through job module its showing 'Running'. But it is not displaying any results or increment in monitor option. It is...
12 April 2021 9,779 0 View
Hello everyone, Here is my situation. I have some nucleotide sequences from which amino acid sequences were predicted using Prodigal. Then an hmmsearch of a target amino acid sequence was...
05 April 2021 1,385 6 View
So I'm using this code to try and control the Keithley 2450 using a 64bit win 10 pc, im running all this in jupyternotebook. The code I'm running: " import numpy as np import pandas as...
02 April 2021 658 0 View
These are initially solitary rod like small structures floating in the media, and then they start forming aggregates. Could this be bacillus? How to deal with it.
30 March 2021 7,733 6 View
Hello, I want to work with some gene expression data that is recorded in TPM. The data has two biological replicates with their own TPM values. How should I go about using this data? is there a...
23 March 2021 8,090 3 View
Hello, Searching for the ultimate housekeeping gene (with expression stability) that works well in human pulmonary fibroblasts (besides GAPDH), any suggestions?
18 March 2021 4,905 4 View
In order to increase humidity to 99% I have to put a container filled with water in side (Thermo Scientific) CO2 incubator. The problem is that due to evaporation of water, water collects at...
16 March 2021 2,247 3 View
Hello, My current lab is analyzing qPCR data in a method that is frankly, very incorrect. Can anyone recommend a step-by-step example for analysis of significant differences between treatments,...
01 March 2021 2,255 0 View
I have question as described in the title - how can I add residues to protein, with the PyMol Builder, and mark them as non-organized? PyMol gives options to add them as beta-sheets or...
28 February 2021 5,693 2 View
I ran RT-qPCR for a control and a treated group and it appears that my gene of interest was not expressed at all in any of the subjects in the control group. So I was wondering if I could...
21 February 2021 6,692 10 View
I've been reading up on how to best represent qPCR data, specially on answers given on this site by Dr. Jochen Wilhelm. I understand box plots are the way to go, but I still don't understand them...
12 February 2021 7,745 3 View
Hi everyone, I'm looking for some help troubleshooting a recent qPCR issue I've encountered. I am using fluorescent Taqman probes and a qPCR master mix that includes ROX as a passive reference....
09 February 2021 643 3 View
We use the NEBNext Library Quant Kit for Illumina to determine our Illumina library concentrations. Before taking the library to qPCR, we usually run the library on Bioanalyzer to get an idea of...
28 January 2021 7,172 4 View
Hello.....I am reconstructing the amplification curves from raw fluorescence data out of the .xml file exported from the LC480II cycler. I cannot understand how to apply color compensation off a...
27 January 2021 922 0 View
Hello together, I am trying to establish the protein-assy by Bradford (1976) following the instructions from "The Protein Protocols Handbook" edited by J.M. Walker. I am struggling with the...
26 January 2021 134 3 View
We're trying to isolate primary intestinal epithelial cells from 13 days old Balb/c mice. Although our protocol allows us to efficiently isolate them with minor fibroblast contamination, when we...
22 January 2021 5,215 0 View
I've been working on growth of sea cucumbers for a decade. Through empirical mark-recapture studies in the field lasting years, other authors (Uthicke et al. 2004) and I have found that large...
11 January 2021 235 5 View
I am looking for isolation (FACS) and cell culture protocols for rat Type I alveolar epithelial cells. I have read "Isolation and Culture of Alveolar Epithelial Type I and Type II Cells from Rat...
10 January 2021 900 0 View
And which solution should I dilute the 1-ABT and how much would be ideal to add in 100ml of culture medium? Considering that I will be in the presence of 1ppm (165ul) of the emerging pollutant...
07 January 2021 6,777 0 View
I am trying to determine what best time-point to harvest my cells, HMVEC, after transduction with long non-coding RNA to determine changes in mRNA levels, specifically VEGFR2/KDR. I am capable of...
20 December 2020 5,084 2 View
Hi, I have been doing Reverse Transcriptase qPCR on some liver mRNA samples. I am targeting liver inflammation genes like Col1a1, alpha SMA, IL6, IL1B and etc... Even though mRNA concentrations...
18 December 2020 6,464 3 View
Hello everyone, I have fabricated a stretchable antenna that uses silver nanowire (AgNW) as a patch and Polydimethylsiloxane (PDMS) as a substrate, but I encountered a problem when installing SMA...
06 December 2020 8,422 4 View
I wonder if the positive control primers and probes authorized by the US CDC for amplifying human RNAse P are specific to the RNAse P chromosomal DNA. My thought process is that if primers are...
02 December 2020 2,633 2 View