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Questions related to Quantitative RT-PCR
I searched the web and found no resources for t-test or other similar tests on fold changes calculated using Pfaffl method. How to compare two groups or even one treatment and one control group?
13 September 2018 4,549 9 View
I am extracting bacterial DNA from food samples for 16S sequencing; processed foods, ready to eat products, meat etc. We have a problem with high levels of host DNA, especially soy DNA, which...
13 September 2018 4,205 4 View
I want to amplify DNA with Internal Control
22 August 2018 492 3 View
I have two different tumor types, Tumor A and Tumor B, with 10 samples for each tumor type. I ran qPCR for a panel of 45 genes and I would like to plot the expression values in a heat map to...
10 August 2018 6,380 4 View
Minimum concentration required.
06 August 2018 4,596 1 View
I would like to answer following questions: - the specific area of brain that Camkiia-Cre would be active in - specific cell types that the KO will occur in - figure out for each cell type (major...
29 July 2018 3,010 0 View
Hi all, As we all know that 16S amplicon seq data by Illumina sequencing is semi-quantitative and therefore I want to normalize my data output with the qPCR 16S data. Can you please recommend me...
27 July 2018 1,925 3 View
is there any publish research show the salivary biomarkers concentration for breast cancer? iam trying to design a salivary biosensor for early detection of breast cancer. recommend me some...
27 July 2018 9,525 3 View
I would like to represent my qRT-PCR results in a bar plot but I am not sure to use SEofdiff or SEM as error bar?
09 July 2018 606 6 View
I have tried to established the amount of RNA input in my RT-qPCRs reactions to amplify interest targets in human monocytes-derived dendritic cells and I perform a RT-qPCR with 4 different RNA...
20 June 2018 3,625 1 View
I am comparing several genes in mutant vs. wild-type samples across multiple plates in qPCR using ddCt. Can I use one of the same wild-type samples (e.g. WT1) on each plate to do interplate...
13 June 2018 1,851 3 View
I did the RTqPCR of a gene in different cell lines samples and I used GAPDH as a housekeeping gene. I calculated the deltaCt and I want to calculate the fold change. What's the difference between...
06 June 2018 2,897 4 View
Hi, After qPCR this odd melting curve with multiple peaks within one big peak appeared in all my samples (attached below). I have performed qPCR on these cDNA samples two times before this run...
31 May 2018 3,329 3 View
Dear all, I am planning a qPCR experiment with multiple samples (4 groups with 7 samples + a dependent control to each sample and an undipendent control group, 7 samples again ) and multiple...
22 May 2018 8,073 6 View
Dear All, I have a few questions pertaining to the RNA immunoprecipitation procedure. 1. How do we decide if we need to include a cross-linking step in RIP? 2. If a cross-linking step is...
16 May 2018 4,234 2 View
We are currently working with murine bone marrow macrophages. We currently use the delta delta CT method comparing our treatments groups to our un-stimulated control cells and are getting very...
15 May 2018 8,391 4 View
I am doing taqman qRT PCR of miRNAs in leukemia patients to access specific miRNA expression pattern by using endogenous control. I got confused with fold change calculations using delta delta ct...
08 May 2018 6,431 5 View
I have used 18S primers as housekeeping genes for my yeast strains. These are showing 1.5-2.0 CT value on RT-PCR while genes under study are showing CT value in higher range from 23-30. I also...
27 April 2018 8,057 9 View
I am a beginner in performing Chromatin Immunoprecipitation. While reading manuals and notes on ChIP and its data analysis , I came across two main methods of interpretation of ones results:...
25 April 2018 4,896 3 View
After synthesising cDNA today I noticed some residue on the seal and on the walls. Most of the residue on the top, on the seal, came down upon centrifugation... However om The walls the tiny...
10 April 2018 9,445 3 View
SOME ELISA kits use ab that recognizes both the human, mouse, and rat cardiac troponin T antigen, Do you know if any troponin t or ı kit that we use in autoanalysers in human laboratories can...
10 April 2018 6,636 0 View
Hello! I have averaged the duplicates of my raw Ct values and have used the delta delta Ct method to get a fold-change for each of the data within each subgroup. Each subgroup has around 4-6 lots...
07 April 2018 8,484 3 View
I have done qRT-PCR for a single gene on leaf sample of tea using 18S rRNA as the house-keeping gene. How do I analyse and interpret the results?
05 April 2018 4,263 3 View
Hello (again), I will write this in as simple terms as possible. I have two diet types, let's call one Normal, the other HF. I have WT and TG mice participating in each diet group. Therefore 4...
30 March 2018 3,989 4 View