Need for PDF opportunity in good University?

I just completed my Ph.D. very recently. I am looking for any PDF opportunity at a foreign university. I have some decent publication in the field of solar pv systems. Can anyone suggest me some...

16 April 2019 7,007 1 View

Applying heat transfer analysis in turbine blade what are the boundary conditions we can use?

I'm now doing my final project about thermal barrier coating in turbine blade. For the optimization of TBC in turbine blade I'm going to make heat transfer analysis.Now I want the boundary...

01 March 2019 2,812 3 View

Can we do RT PCR with basal p53 expression?

I have extracted RNA from tumor tissue sample and wondering whether the basal level of p53 expression enough to do RT PCR amplification using p53 transcript specific primers? Thank you

20 September 2018 3,669 3 View

Can anyone suggest model antigen-antibody pairs (best studied models for direct and sandwich immunoassay)?

We have developed a fiber optic sensor and we wish to evaluate them for biosensing. Is there any standard antigen-antibody pair for immunoassay, namely direct and sandwich formats. For example...

18 December 2017 6,541 3 View

Suggest any suitable technique for making 6mm dia. hole on ~4mm thickness fiber composite without any delamination.

19 June 2017 928 7 View

What is the abbreviation of TECS (It is a hard fluoropolymer used as clading in silica optical fibers)?

05 April 2017 4,495 2 View

Can anyone suggest better protocol for extraction of yeast RNA?

03 October 2016 8,147 3 View

Microfiltration for enzyme liquid?

I want to achieve a microclear (free of any bacteria & fungi) enzyme liquid, below are parameters of my feed Feed Sample: Papain Enzyme liquid pH: 4.5 to 5.8 Total Suspended Solids: 9% to...

22 July 2016 6,546 1 View

P53 mutation status of MX-1 breast cancer cell line?

Is MX-1 breast cancer cell line P53 wild type or mutant?

28 January 2016 2,433 3 View

Which will be the most suitable medium to culture MX-1 breast cancer cell line?

24 November 2015 8,377 2 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Can we recognize signs of the "volatility clustering" property of a time series in its time domain plot?

My purpose is to recognize the volatility clustering property without statistical tests such as The Lagrange Multiplier (LM) test.

24 February 2021 4,150 3 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Could you please suggests a tutorial or paper or any other materials to learn multiple gene expression in TCGA and GTEx databases?

I would like to analyze multiple gene expressions in normal and cancer tissue by using the data in the TCGA and GTEx databases. Please suggest a tutorial or paper showing step by step analysis of...

23 February 2021 9,007 3 View