Old question, new answers: what are your protocols and best practices for acquiring high quality, intact RNA for downstream analyses?

20 October 2022 0 2K Report

Hi everyone! I would like you to show off your most successful strategies to acquire bacterial / archaeal high quality intact RNA from environmental samples / mixed community (liquid sample)

Thanks!

Artur

Badges
Science method

More Artur Zaduryan's questions See All

How to fix a contour legend in ANSYS APDL?

Hello everyone! During a postprocessing of thermal analysis done in the Ansys Mechanical APDL I'm facing the problem with Legend appearance plotting the temperature field. While background is...

26 March 2024 9,843 0 View

ONT Nanopore (MinION): 16S rapid kit or Native kit for... 16S analysis?

Hey everyone, my question is maybe strange at first glance, but simple: is the rapid 16S kit's only real advantage the significantly larger 16S data amount generation? Shouldn't I be perfectly...

12 March 2024 3,909 2 View

Na-DL-lactate in a bacterial growth medium - what is the role/function?

Hi! I am prepping a sulfate reducer (Desulfovibrio sp.) medium (M63 from DSMZ https://mediadive.dsmz.de/medium/63) and I am trying to make sense of the formulation. So far my understanding...

12 February 2024 587 4 View

How do you think recent events in OpenAI will affect further development of AI, especially ChatGPT, and its applications in Academia?

For details on the current OpenAI leadership situation see e.g. https://www.theguardian.com/technology/2023/nov/19/openai-investors-push-for-return-of-ousted-ceo-sam-altman-chatgpt

18 November 2023 9,274 5 View

Researchgate has just turned 15 years old. Would you please share your comments, wishes and remarks on this anniversary?

RG now has more than 25 millions of users worldwide as announced at https://twitter.com/ResearchGate/status/1659204067066019841 see also...

28 May 2023 3,812 10 View

What features of constructivism, as a theory in contemporary international relations, would you apply to the evaluation of the Three Seas Initiative?

Describing Three Seas Initiative in the light of constructivism should base on an examination of regional cooperation in the CE region in the context of two types of knowledge: knowing-that and...

28 May 2023 9,820 1 View

Could you please share your recent Research Spotlight works here?

Just in case, here is a description of Researchgate's Research Spotlights feature https://help.researchgate.net/hc/en-us/articles/14293040489745

04 May 2023 8,270 1 View

Will you please share your favorite Researchgate projects before they go away on March 31?

Please share here the links to your favorite Researchgate projects, be it your own or somebody else's, before they go away on March 31. To get things started, here's the link to my own project...

27 March 2023 9,989 3 View

What's new in the project Multidimensional Integrable Systems?

This is to provide updates on my research as a backup communication channel for my project Multidimensional Integrable...

23 March 2023 1,014 7 View

The end of Researchgate projects: what to do?

Do you know of any good non-intrusive ways of communicating updates to your research within the ResearchGate site to mimic the project update feature? This question arises as Researchgate will...

23 March 2023 1,311 16 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to prepare the nanoparticle treated fungal sample for Environmental SEM analysis?

A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.

07 August 2024 5,307 1 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View