Hi everyone! I would like you to show off your most successful strategies to acquire bacterial / archaeal high quality intact RNA from environmental samples / mixed community (liquid sample)
Thanks!
Artur
Hello everyone! During a postprocessing of thermal analysis done in the Ansys Mechanical APDL I'm facing the problem with Legend appearance plotting the temperature field. While background is...
26 March 2024 9,843 0 View
Hey everyone, my question is maybe strange at first glance, but simple: is the rapid 16S kit's only real advantage the significantly larger 16S data amount generation? Shouldn't I be perfectly...
12 March 2024 3,909 2 View
Hi! I am prepping a sulfate reducer (Desulfovibrio sp.) medium (M63 from DSMZ https://mediadive.dsmz.de/medium/63) and I am trying to make sense of the formulation. So far my understanding...
12 February 2024 587 4 View
For details on the current OpenAI leadership situation see e.g. https://www.theguardian.com/technology/2023/nov/19/openai-investors-push-for-return-of-ousted-ceo-sam-altman-chatgpt
18 November 2023 9,274 5 View
RG now has more than 25 millions of users worldwide as announced at https://twitter.com/ResearchGate/status/1659204067066019841 see also...
28 May 2023 3,812 10 View
Describing Three Seas Initiative in the light of constructivism should base on an examination of regional cooperation in the CE region in the context of two types of knowledge: knowing-that and...
28 May 2023 9,820 1 View
Just in case, here is a description of Researchgate's Research Spotlights feature https://help.researchgate.net/hc/en-us/articles/14293040489745
04 May 2023 8,270 1 View
Please share here the links to your favorite Researchgate projects, be it your own or somebody else's, before they go away on March 31. To get things started, here's the link to my own project...
27 March 2023 9,989 3 View
This is to provide updates on my research as a backup communication channel for my project Multidimensional Integrable...
23 March 2023 1,014 7 View
Do you know of any good non-intrusive ways of communicating updates to your research within the ResearchGate site to mimic the project update feature? This question arises as Researchgate will...
23 March 2023 1,311 16 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.
07 August 2024 5,307 1 View
Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.
07 August 2024 8,782 3 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View