Where can I get LN-308 cell line?

Hi! I am looking for a cancer cell line with endogenously high expression of GPR55. LN-308 seems to have the highest level of GPR55 among multiple cancer cell lines (according to Genevestigator;...

20 October 2020 3,004 3 View

How can I quantify bacteria viable cells in a non-sterilized organic compost?

I'm working with the enrichment of an organic compost with PGPR, but this compost is not sterilized. How could I quantify viable cells of Azospirillum brasilense, Bacillus subtilis, and...

01 April 2020 9,486 4 View

How to model near-stall compressor operation with RANS CFD?

Hello everyone. I am looking into near-stall behaviour of the multistage axial compressor using CFD with RANS method. What is the best practice to establish if the compressor is close to stall,...

09 March 2020 8,847 8 View

What iron species are precipitating after autoclaving ferric chloride water solution?

I made 10mM FeCl3 solution in MQ water. After autoclaving it (standard 15min procedure) solution turns orange-brown. Filtering using 0.22um filter does not remove the color (but some of it is...

24 September 2019 2,407 7 View

What is a typical RFU value for a "low concentration" standard sample when quantifying RNA with Quantus Fluorometer?

Hi, quick question, What typical RFU value should I expect for a "low concentration" standard sample when quantifying RNA with Quantus Fluorometer? I made two standards (100 uL dye buffer, 98 uL...

10 April 2018 5,090 3 View

RNAprotect reagent formulation?

Hello, For the project I am currently involved in, I am in need for a cheap, non-toxic, pH 7+, non-disruptive buffer for RNA stabilization of bacterial cells (applied to a large environmental...

04 March 2018 5,089 14 View

How much does ammonium sulfate precipitation affect nucleic acid integrity?

Hello, In my current project I am at present trying to tackle an issue of rapidly stabilizing bacterial RNA in a large environmental sample. Most known stabilization reagents are not applicable in...

04 March 2018 1,589 3 View

SUOMI-NPP VIIRS DNB (Day-Night Band) radiance data and Light Pollution Assessment

We are working with SUOMI-NPP VIIRS DNB (Day-Night Band) radiance data, which has units of W cm-2 sr-1. The case study area is a bird sanctuary and an inhabited territory that is also used for...

14 May 2017 7,715 2 View

Is there a relation between Hadamard product and standard matrix product?

05 July 2015 5,366 7 View

Can we get the sufficient number of intensity measurements for a complex vector reconstruction in quantum tomography?

In a paper arXiv:1312.0158 the authors proved that any complex vector (thus also the quantum state vector) can be uniquely determined (up to a global phase factor) by 4N-4 generic intensity...

22 June 2015 7,125 4 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View