Contact experts in Quantitative RT-PCR to get answers
1,124 views 901 posts
Questions related to Quantitative RT-PCR
I have very few copper mesh to isolate my InVivo ephy implants and I'm trying to find a distributor in Europe but still not successful found one that sells small amount of it. The item reference...
14 September 2021 3,896 0 View
I am experimenting degradation of polystyrene nanoparticle using TiO2 and I face higher final TOC values than initial TOC. I assume it is due to the generation of CO2 while mineralization. However...
15 August 2021 4,139 5 View
I am having trouble with Nanog IHC in ESCs and iPSCs. In all of the cell lines that I am characterizing, I have measured mRNA expression of Oct4, Sox2, and Nanog, and I have successfully stained...
19 July 2021 3,433 1 View
I have cDNA samples extracted from 90+ individuals which I intend to utilise in a series of qPCR experiments to assess the influence of a specific SNP genotype/s on target gene/s expression. I...
09 July 2021 2,968 3 View
I want to isolate extracellular DNA from 1L of environmental sample (lake water). Is it possible to concentrate the ext. DNA in sample using anion exchange columns ? What are important...
21 June 2021 7,607 0 View
Hi folks I published a couple of things in Nature Education Knowledge and they were well received; but they stopped producing new content. Is anyone aware of similar educational outlets? I'm...
08 June 2021 1,460 0 View
Explain the unequal control and treatment group effect on choices of econometric methods
08 June 2021 6,377 5 View
Good day great scholars. Please I would appreciate help on how to install Discovery Studio and PyRx in Ubuntu. I will also like to know how I can assess/identify the path of the License Pack for...
27 May 2021 9,762 10 View
I am doing a Chip-seq experiment on one human sample and testing the DNA segment I got from Chip by qPCR first. What I found is my primer designed based on UCSC Genome Browser doesn't work very...
19 April 2021 2,388 2 View
Hello everyone, Here is my situation. I have some nucleotide sequences from which amino acid sequences were predicted using Prodigal. Then an hmmsearch of a target amino acid sequence was...
05 April 2021 1,329 6 View
These are initially solitary rod like small structures floating in the media, and then they start forming aggregates. Could this be bacillus? How to deal with it.
30 March 2021 7,666 6 View
Hello, I want to work with some gene expression data that is recorded in TPM. The data has two biological replicates with their own TPM values. How should I go about using this data? is there a...
23 March 2021 8,016 3 View
In order to increase humidity to 99% I have to put a container filled with water in side (Thermo Scientific) CO2 incubator. The problem is that due to evaporation of water, water collects at...
16 March 2021 2,185 3 View
Hello, My current lab is analyzing qPCR data in a method that is frankly, very incorrect. Can anyone recommend a step-by-step example for analysis of significant differences between treatments,...
01 March 2021 2,201 0 View
I ran RT-qPCR for a control and a treated group and it appears that my gene of interest was not expressed at all in any of the subjects in the control group. So I was wondering if I could...
21 February 2021 6,452 10 View
I've been reading up on how to best represent qPCR data, specially on answers given on this site by Dr. Jochen Wilhelm. I understand box plots are the way to go, but I still don't understand them...
12 February 2021 7,524 3 View
Hi everyone, I'm looking for some help troubleshooting a recent qPCR issue I've encountered. I am using fluorescent Taqman probes and a qPCR master mix that includes ROX as a passive reference....
09 February 2021 530 3 View
We use the NEBNext Library Quant Kit for Illumina to determine our Illumina library concentrations. Before taking the library to qPCR, we usually run the library on Bioanalyzer to get an idea of...
28 January 2021 7,106 4 View
We're trying to isolate primary intestinal epithelial cells from 13 days old Balb/c mice. Although our protocol allows us to efficiently isolate them with minor fibroblast contamination, when we...
22 January 2021 5,150 0 View
I am looking for isolation (FACS) and cell culture protocols for rat Type I alveolar epithelial cells. I have read "Isolation and Culture of Alveolar Epithelial Type I and Type II Cells from Rat...
10 January 2021 834 0 View
I am trying to determine what best time-point to harvest my cells, HMVEC, after transduction with long non-coding RNA to determine changes in mRNA levels, specifically VEGFR2/KDR. I am capable of...
20 December 2020 4,969 2 View
Hi, I have been doing Reverse Transcriptase qPCR on some liver mRNA samples. I am targeting liver inflammation genes like Col1a1, alpha SMA, IL6, IL1B and etc... Even though mRNA concentrations...
18 December 2020 6,336 3 View
I wonder if the positive control primers and probes authorized by the US CDC for amplifying human RNAse P are specific to the RNAse P chromosomal DNA. My thought process is that if primers are...
02 December 2020 2,500 2 View
I have seen COVID19 test LoD ranging from 180 to 600000 NAAT Detectable Units/mL So a rapid antigen test with LoD of TCID50 1.13 x10^2 per mL and RT PCR TCID50 1x10^-2 per mL (600 NDU/mL) and a...
29 November 2020 1,379 3 View