Contact experts in Quantitative RT-PCR to get answers
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Questions related to Quantitative RT-PCR
Howdy all! I wanted to enquire as to the possible cause of magnetic bead contamination in final sample elution’s while using the ZymoBiomics MagBead RNA kit. We are doing many extractions in...
09 May 2023 4,674 0 View
Currently I am doing RNA immunoprecipitation to find the targets of an RNA binding protein. I am unclear about the reverse-crosslinking protocol. I have found two approaches one in which they wash...
04 May 2023 9,758 2 View
Warm greetings everyone !! This message is regarding leeds for statisticians who can work in qualitative research/ instrument development. We have completed phase 1 of our project on...
03 May 2023 7,793 2 View
Preventive nutrition: disease-specific dietary interventions for older adults
01 May 2023 4,748 1 View
We performed several PCR assays to detect virulence factor of pathogenic strains, but a faint band amplified on negative control. After this, we have changed all reaction components (one by one)...
27 April 2023 6,621 3 View
If it's the case, what were the proliferation and cell growth status before you realized that it was due to a Mycoplasma infection? Thank you in advance.
12 April 2023 8,824 0 View
I've performed a colony PCR with two unknown bacteria (in triplo). Lanes 6, 7 and 8 shows one bacteria with a nice outcome. The band that I expect is 1465 bp, because during the colony PCR, I use...
06 April 2023 7,069 10 View
cell culture: HEK293F medium: BalanCD, without antibiotics
31 March 2023 8,838 1 View
I'm doing PCR and qPCR frequently. My assay is extremely sensitive so I need to pipette a very exact volume from my SOP. From my experience and also my school lab course, I learned to press the...
30 March 2023 981 4 View
I am trying to publish a journal but most of the ones I have come across are expensive.
27 March 2023 9,672 5 View
When we do a nucleotide blast for certain sequences, there are tons of results like "cloning vector" or "synthetic construct". These are plasmids made by researchers. I want to exclude all these...
26 March 2023 4,551 3 View
Does DMSO can have cytotoxic effects on microorganisms and may interfere with the results of microbiological assays? What is the concentration that is used to secerning the antimicrobial activity...
23 March 2023 6,679 3 View
Hello! Has it happened to you that the fetal bovine serum is contaminating and eventually (not immediately) your cells become contaminated? If so, what did you do to fix the problem? Thank you!!!
23 March 2023 2,057 5 View
I'm a PhD student working on protein engineering & immunology and making recombinant proteins in lab for work with mammalian systems. I have tried using ClearColi previously (BL21 DE3 w/...
22 March 2023 5,718 3 View
I need to compare data of two ratios. The ratio obtained by treatment : control reading from qPCR. I am not sure whether the conventional t test would be ok to determine significance between two...
16 March 2023 1,572 2 View
I have a set of data from three groups across 3 time points (2 groups are subjected to an intervention and the third acts as control). I want to evaluate intra and inter-group differences between...
09 March 2023 6,726 3 View
Anyone knows name of a journal for cancer research where I can publish freee of cost or low cost.
07 March 2023 3,689 2 View
Guillermo Zalba is not part of the team of the article, plese delete his name Best regards Alejandra Guillermina Miranda-Díaz, MD, PhD
02 March 2023 7,251 2 View
I am analyzing data set from a Glucose Sensitivity test. In order to show the glucose tolerance between time point 0 and time point 120, I intend to use the Area Under the Curve (AUC) feature on...
27 February 2023 6,698 0 View
I'm a little confused, I am doing a PCR reaction that requires 100ng of genomic DNA template, and the concentration of my genomic DNA is 591,9ng/ul. So is 100ng of the template the same as saying...
26 February 2023 9,433 3 View
What factors affect melt peak [-d(RFU)/dT) in real-time PCR? What should I do if I want to increase my melt peak [-d(RFU)/dT)?
23 February 2023 179 2 View
I've done a qPCR reaction using Bio-Rad CFX Duet, 10 ul reaction, using EvaGreen dye. as you can see, image 01 is what I think as a good amplification plot. Today I do the qPCR again using a...
17 February 2023 4,224 3 View
I have four groups, the anova accepted the hypothesis of equal means. But when I used the two sample t-test 6 times (4 combination 2), two of these 6 tests are significant, which method we prefer?
16 February 2023 6,960 1 View
What is main difference between Ct and Cq value? When do we use Ct value and when Cq In Real Time PCR? How to determine ct/cq value in a given qPCR assay?
15 February 2023 5,188 5 View