I did prepare 1litre of comet assay lysis solution by adding sodium lauryl sarcosinate. For the assay used only 100 or 150ml and kept the remaining at 4 degree celsius for future use. After 2 days, white crystal precipitate has started to appear.
Hello Ameena Stellixir
Sodium lauryl sarcosinate precipitates with long term storage at 4°C. So, you should store the lysis solution containing sodium lauryl sarcosinate at room temperature and not at 4 degree celsius.
Best.
Ameena Stellixir, I have always stored such a solution at room temperature and so I never experienced such a problem. I would suggest you should warm the lysis solution at 40-45 degree C in the water bath for a few minutes. Maybe this might solve the problem. Do not filter the solution.
If the lysis solution has precipitated, it is likely due to the presence of excess salt or other contaminants. One solution could be to filter the lysis solution using a sterile filter to remove any particulate matter or crystals. Alternatively, you could try warming the solution to room temperature or gently heating it to dissolve the precipitate. It may also be necessary to adjust the pH of the solution if it is too acidic or basic, as this can also contribute to precipitation. If none of these approaches work, it may be necessary to prepare a new batch of lysis solution.
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