Which loading control is better for brain tissues in western blot?

I need to detect protein in the hypothalamus of brain. As a loading control which one would be better?

06 July 2015 5,290 2 View

Can someone explain why EDTA needs basic condition for dissolving?

EDTA is insoluble in water but dissolve with the increment of pH. Could you please explain how pH works for dissolving EDTA?

08 September 2014 6,873 10 View

How can I load equal volume in each lane during gel running of Western Blot?

07 August 2014 4,787 7 View

How does one calculate protein concentration using formula?

OD is 0.36, what has been got using Lowry method. Is there any formula to calculate protein concentration?

07 August 2014 1,554 29 View

How can you get absolute quantification using standard curve for qRT-PCR?

I have standard curve and know the plasmid size. How can I get the copy number? Can anyone explain details or give reference? It will be really great help.

02 March 2014 4,161 3 View

What is a good Gbs extension?

I have downloaded a mouse chromosome from the NCBI database, which is a gbs file. I opened it in notepad (windows) to see my file, unfortunately all of the document's looking in that file are...

02 March 2014 6,006 2 View

How can I analyse data for absolute quantification, relative quantification and PCR efficiency using standard curve of plasmid?

I am very new in qRT-PCR.

01 February 2014 1,783 3 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

How to remove DCU and HOBT from my reaction?

I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??

28 February 2021 2,153 1 View

What is the most common number of epitopes in an multi-epitope Vaccine?

hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...

27 February 2021 3,882 3 View

How to increase solubility of Poly acrylic acid in Dioxane solvent?

I am working with Polyacrylic acid (100.000Da) and instead of water, I need to use another solvent (dioxane) to dissolve PAA. However, because of the high molecular weight, it's quite hard to...

25 February 2021 2,225 5 View

Why is NH4OH added in heavy metals limit test?

The limit test I'm referring to is USP method II. In this procedure you need to dropwise add 6N of NH4OH to the sample until it the pH is alkali. After that you need to dilute and add HOAc until...

23 February 2021 2,049 3 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

Is this the write procedure for acid digestion of soil for heavy metal analysis using AAS?

How to do acid digestion of soil sample with 3:1 aquaregia in an open vessel?

23 February 2021 3,053 7 View