How and when Na/K ions get separated from Nucleic acid during DNA extraction?

More S M Majedul Karim's questions See All

Which loading control is better for brain tissues in western blot?

I need to detect protein in the hypothalamus of brain. As a loading control which one would be better?

06 July 2015 5,339 2 View

Can someone explain why EDTA needs basic condition for dissolving?

EDTA is insoluble in water but dissolve with the increment of pH. Could you please explain how pH works for dissolving EDTA?

08 September 2014 6,951 10 View

How can I load equal volume in each lane during gel running of Western Blot?

For 30 µg protein loading in each lane, concentration of one protein is 1.97 µg/µl and another is 1.41 µg/µl, that means for first one would be 15.2 µl and 2nd one is 21.2 µl. How to load equal...

07 August 2014 4,862 7 View

How does one calculate protein concentration using formula?

OD is 0.36, what has been got using Lowry method. Is there any formula to calculate protein concentration?

07 August 2014 1,611 29 View

How can you get absolute quantification using standard curve for qRT-PCR?

I have standard curve and know the plasmid size. How can I get the copy number? Can anyone explain details or give reference? It will be really great help.

02 March 2014 4,239 3 View

What is a good Gbs extension?

I have downloaded a mouse chromosome from the NCBI database, which is a gbs file. I opened it in notepad (windows) to see my file, unfortunately all of the document's looking in that file are...

02 March 2014 6,049 2 View

How can I analyse data for absolute quantification, relative quantification and PCR efficiency using standard curve of plasmid?

I am very new in qRT-PCR.

01 February 2014 1,833 3 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

Determining the worth of a point improvement in Hamilton Depression Scale?

Dear readers, Thanks for your attention. I am wondering about the health economic problem of quantifying the value of interventions which a) prevent, b) improve symptom profile and c) ultimately...

05 August 2024 3,246 1 View

What is the best blank for nanodrop if I want to read a recombinant protein concentration?

Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC

01 August 2024 967 3 View

Conjugation of PEG-Amine to an Amino Acid Using EDC?

I am attempting to conjugate PEG to an amino acid at the C-terminus, for the purposes of producing nanoparticles. I have been told that PEG modified with amine groups can be used for this purpose,...

31 July 2024 2,033 1 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

Is it possible to use a safer acid instead of monochloroacetic acid to convert bacterial cellulose to carboxymethyl cellulose?

Hi, researchers, Is it possible to use a safer acid instead of monochloroacetic acid to convert bacterial cellulose to carboxymethyl cellulose?

29 July 2024 4,570 0 View

My cfDNA extraction protocol using silica beads works with plasma collected in COD SAGM bags but not with plasma collected in K2 EDTA tubes. Why so?

I am currently performing cell-free DNA (cfDNA) extraction from plasma using an in-house developed kit that employs silica-coated magnetic beads. My protocol works effectively with plasma obtained...

28 July 2024 3,559 0 View

What is the Principle of Cysteine-carbazole-sulfuric acid reaction with glucose?

I have been looking for the principle of Cysteine-carbazole-sulfuric acid reaction in research papers and books, but couldn't find it. This reaction is mainly used for the quantification of...

23 July 2024 3,891 0 View

Can sulfuric acid be used to prepare anthrone reagent when it has oxidized and changed color in reaction to light?

Sulfuric acid has changed color to yellow.

16 July 2024 6,964 0 View

Qiagen circulating nucleic acid kit poor yield?

Hi, Attempted to extract cfDNA from a 4 mL plasma sample, with poor yield. Went through the manual in the section regarding poor yield, and feel that this does not apply. Some potential...

14 July 2024 7,475 1 View

Janarthanan P.

Hello,

While you doing alcohol precipitation DNA get precipitate, Remaining other Na/K ions dissolved in alcohol. Finally you will get purified DNA

Adam B Shapiro

I think you end up with the Na+ or K+ salt of DNA, not the free acid.

S M Majedul Karim

I have got two different comments from two researchers. is there anyone who help me to draw a conclusion?

Andrew Reed

I would agree with Adam. I don't believe the alcohol precipitation would remove the salt. You could use a Sep-Pak C18 column to bind and wash the DNA, then eluting with Methanol. This should remove any salt if you are concerned.