I plan to use next generation sequencing of RNA and DNA on clinical samples of whole blood (EDTA), after storing them for weeks-months at 0-80C what is the best method to store whole blood to minimise RNA degradation? extracting nucleic acids prior to freezing appears to be one option or using Trizol another options, does anyone have any advice? Many thanks.
Dear Rebecca ,
Best method to store EDTA derived samples is to convert them in to WBC pellets as whole blood will get haemolysed on storage at -80 degree. You can store them in liquid Nitrogen also.
Thank you, I have been looking at the liquid nitrogen option but otherwise separating off the plasma and WBC does seem the way to go. Thanks for your help.
Through different collaborations, I have worked with frozen WBC pellets. As Shefali mentions, this is doable, but your DNA yield will probably be higher if DNA is extracted from the WBC straight away. I have had some trouble with DNA yields from WBC pellets in the past, so if direct DNA isolation is an option I think that is preferable.
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