I am processing 400 um hippocampal slices immunohistochemically. I am incubating the slices in the primary antibody solution overnight at 4 degrees C and the secondary antibody and nucleic acid stain solution for 6 hr at 4 degrees C. At present, these incubation conditions have not been performed under agitated conditions. Is it necessary to use gentle agitation during these incubation stages?

More Spencer G. Smith's questions See All
Similar questions and discussions