I am trying to isolate isoforms of plasmids from agarose gels using a nucleic acid purification kit, and I have been playing around with the my choice elution buffer in order to see if I can increase the yield and purity of my products. I haven't seen anything about using TE buffer for elution, and as far as I know, Tris-HCl is the standard. In my most recent attempt at extraction, I managed to obtain highly pure DNA, but with a lower yield than I had wanted.
Is TE buffer a bad choice for elution?
TE will no affect your elution, but it will most likely also not increase your yield. If you want to use the eluted DNA for PCR, the EDTA in the elution buffer could be a problem, unless you dilute the DNA before using it as PCR template.
No. It is alright to use TE. Most elution buffer are Tris EDTA . Having EDTA in the solution in fact protect the DNA from being degraded. Of course the concentration has to be right . The low yield that you have is most likely from the isolation protocol. We find that for some cell types, we need a lot more cells than the recommended number in the protocol.
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