Last night, the fridge (-20°C) in our laboratory didn't work well. It was not cooling and the temperature rose up to room temperature. The DNA ligase kept in that fridge. Nobody notices until next morning.
Is it still okay to use that enzyme?
For my IFA, I am using Alexa Fluor conjugated secondary antibodies. In my lab, other antibody, which is Cy3 conjugated has been kept at - 20 °C in aliquots. However, on the company data sheet, it...
22 January 2024 5,212 3 View
In my experiment, I use a plasmid (~16kb). What I want to do is to delete Luciferase gene from that plasmid by using a specific pair of primers with PCR technique. Last time, I tried and I got no...
22 May 2022 5,745 3 View
I have read an article in which the author mentioned that the cell lysates were mixed with the primary antibody before loading into the gel, then transferred to the membrane and probed with the...
30 September 2021 9,117 2 View
When AAV is produced in HEK cells, the helper gene, plasmid carrying packing system, and trans gene are used. AAV carrying trans gene is produced in a package. But, we also use AAV as a plasmid in...
09 June 2020 1,603 0 View
MTT dye is reduced by oxygen reductase of the nucleus of living cells. Then Why does we add DMSO ?
08 May 2018 9,640 3 View
Hello everybody, I would like to ask you any protocols for Dengue Virus Propagation. Thanks.
08 May 2018 6,907 2 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
It has been documented that apoptotic cells themselves can induce phosphorylation of serine 139 on H2AX (γH2AX) due to DNA fragmentation during apoptosis (doi: 10.1074/jbc.275.13.9390). As γH2AX...
28 July 2024 7,983 2 View
Hi, Now I am doing a single-base editing by using HiFi Cas 9, guideRNA, single stranded DNA, HDR Enhancer V2 and my tranfection mehtod is lipofection (RNAiMax). Unfortunately, after gene editing,...
26 July 2024 2,136 0 View
Hello, We found three packages of Illustra™ MicroSpin™ G-25 columns in the cabinet of an unused lab. They are very old but have never been opened. I have never used this kit before, and I couldn't...
25 July 2024 4,927 3 View
Using INTRON's DNA-spin Plasmid DNA purification kit to extract plasmids from specific E.Coli strains, my team and I have encountered numerous plasmid dimerizations that lead to over-weighted...
23 July 2024 3,490 6 View