I want to sequence the amplicons with two different library prep kits.
1. Nextera DNA Flex Library Prep. kit
2. QIAseq Ultralow Input Library Kit
Can I use the libraries from both preparation kits in a single cartridge simultaneously? If not, what is the possible reason?
Furthermore, is it possible to use the Adapters of one library prep. kit for another?
Hi Zohaib Ul Hassan ,
You can use libraries made from different lib prep kit into single flow cell if
1) they both are of same read length.
2) the kit contain Illumina compatible adapters.
If your libraries have different data requirement, you need to adjust the loading volume of libraries accordingly.
The question is why you want to do that? These kits have different purposes ... anyway, you can use different kits as long as you’re using compatible adapters.
Good luck
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