Oil extract measuring?

I want to ask a question about oil extracts. How to prepare extract for researches. For example I want to measure antioxidant activity. Oil won't be dissolved in water if to dilute the studied...

01 November 2018 8,970 2 View

Which type of statistical analysis should I use for analysing Likert-type qustions in modelling user behaviour?

07 April 2017 9,817 6 View

Which sampling technique should I use to survey the UK academic staff?

14 March 2016 2,742 11 View

Can Ab-magnetic beads used for MACS hinder further detection of antigen with flow cytometry?

Hellow everyone! I am using eBiosciense magnetic beads kit for MACS separation of murine CD11b+ cells (= monocytes and granulocytes). It consists of Biotin-labelled anti-CD11b Ab; and a secondary...

04 November 2015 1,299 6 View

Why do I get these autofluorescence peaks in in flow cytometry?

Hi all, I've just found something interesting about autofluorescence in flow cytometry. I've found, that autofluorescence, especially on FL1 [ex 488 / em 533] and FL2 [ex 488 / em 585] channels is...

15 September 2015 8,267 3 View

How to increase anatase titania conductivity experimentally?

I have titania films growth by ALD and current resistivity is very high (typical 4pp method gives error and multimeter measurements give MOhms values). What experimental methods can be used to...

22 April 2015 3,300 5 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Why am I not able to use all VEP programs for the in silico analysis of avpr1b SNP's?

Good day researchers I am busy analysing the predicted effects of certain SNP's in AVPR1b. When using the VEP prediction programs (SIFT, Polyphen, FATHMM, LRT, Provean, Mutation Assessor, and...

28 February 2021 5,197 1 View

What is the difference between genotype and haplotype data in the ped format?

Hi everybody In the ped format for genotype, alleles of any SNP are represented by two columns (one for each allele, separated by a space). Is a column sufficient for the haplotype to...

27 February 2021 1,965 1 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View