What should be the endpoint (in the context of tumor size) of an experiment in which the animals are injected with CT26 cells? The idea is to measure the tumor until the endpoint, at which point the mouse bearing the CT26 tumor will be sacrificed? Generally, when we work with MC38 or B16, our endpoint is when the tumor size approaches 2cm x 2cm.
Currently working with 5 cell lines, B16 and CT26 being two of them. For CT26 I once harvested the tumors for down stream analysis at sizes 15mm by 14mm because at that point mice overall health appeared compromised.
Currently working with 5 cell lines, B16 and CT26 being two of them. For CT26 I once harvested the tumors for down stream analysis at sizes 15mm by 14mm because at that point mice overall health appeared compromised.
You should not go over a "tumor area" greater than 450 mm² if you actually want to fit with ethical standards.
We prefer to use the CT26 model as orthotopic grafts into the caecum, which is a model by far closest to the clinical reality than a s.c. colon cancer model.
Best regards
Robert
Thank you, Tareq for information.
Thank you, Robert, for the suggestion and the additional information. I am new to this model and this information will be very helpful.
I really appreciate your help.
- Badges
- Science topic
- Similar topics
- Medicine
- Disease
- Disease Models
- Animal Models
- Rodent Models
- Mouse Models