Currently, I’m working with model of gastric ulcer induced by EtOH or NSAIDs (diclofenac). I use ImageJ software for macroscopic scoring to compare the ulcer area. Unfortunately, I couldn’t find any reliable system for microscopic scoring.
Could you recommend any scoring system to use in microphotographs (H&E staining) obtained from mice stomachs?
Hi Dr Zatorski,
Currently,i am researching on gastric ulcer model induced by acetic acid in rat. I use Image J for assessment of macroscopic and microscopic ulcer area as well as healing effects evaluation .you can find more information in attached articles.
Good Luck,
Thank you Dr Hosseini for useful articles.
Finally, I have decided to use method described by Shimoyama et al. in article:
Shimoyama, A.T., Santin, J.R., Machado, I.D. et al. Naunyn-Schmiedeberg's Arch Pharmacol (2013) 386: 5. doi:10.1007/s00210-012-0807-2, wich description you can find below.
Histological analysis:
After macroscopic analysis, a small portion of each stomach was fixed in 4 % formalin solution, dehydrated through increasing grades of alcohol, and embedded in paraffin. Five-micrometer sections were made using a microtome, and stained with hematoxylin-eosin solution. Tissue preparations were observed and microphotographed under a light Axioplan 2 Zeiss microscope. Leukocyte infiltration was assessed microscopically (×100), with counts being made in ten fields per specimen.
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