Hi! This protocol has been used in my lab to handle healthy rat liver samples (see below). But now, i'm going to start a project for quantifying membrane proteins in healthy and diseased human liver tissue and this doesn't feel like a safe way of doing it.

Has someone homogenized Human liver before that could share a few tips and maybe the protocol they followed?

thank you!!!

Isolation of membrane protein

Rat liver and kidney weighing were homogenized using glass dounce homogenizers in Sodium Bicarbonate with protease inhibitors. After brief centrifugation, the clear supernatant was incubated with Sodium Carbonate at 4 oC for 15 minutes. Samples were centrifuged at 100,000g for 1 hour at 4 oC. The pellet was resuspended in1xPBS containing protease inhibitors and disrupted briefly by ultrasonication. The protein was quantitated by Pierce BCA Protein Assay kit.