I am working on the assessment of the efficacy of several new vitamin D analogus in treating postmenopausal osteoporosis in rat model. Postmenopausal osteoporosis was induced by performing ovariectomy (OVX) in 3 months old female rats. By literature review, there have been two kinds of setting for initiation of drug administration: 1. Drugs are used just after OVX operation or a week later for wound healing. 2. After OVX operation, the rats are kept for 12 weeks to induced obvious osteoporosis. Then, drug administration begins. Most literatures took the first setting, setting 2 is also adopted by some studies. It seems that setting 1 is focusing on the protective effect of drugs on bone mass whereas setting 2 is mainly on the ability of drugs in correcting already established osteoporosis. I am wondering which one is more rational and suitable for my experiment design.
Good morning, congratulations for the study.
The choice of method depends on your goals. Note that for studies of osteoporosis, it is extremely important that the animal has reached its peak bone mass, an event that occurs around the ninth month of life of the animal.
As you study vitamin D, and as we know the difficulty of reverting osteoporosis, also thinking of public spending, all preventive therapies are welcome in this way, I believe to be the most consistent setting 1, where you study the prevention of bone loss and not treating the disease already installed, but I would point out again that all depends on your goal.
Best regards
A problem of ovx rat model - ResearchGate. Available from: https://www.researchgate.net/post/a_problem_of_ovx_rat_model#57e3c7c596b7e45c812e42fd [accessed Sep 22, 2016].
I agree with Camila as it mostly depends on your study goals. I would suggest that you try both models as prophylactic and remedial therapies, depending on your available resources and fund, and see how it goes to reach a firm conclusion about the performance of the new analogues. At the end, these new agents could provide both preventive and therapeutic approaches for a common health problem and you will never know their potential except by trying both models.
Wishes,
Bassem
I agree with both. You should see both the prophylactic and treatment role of the drug. In some settings it is also compared with some standard drug. You can also amke other groups in which u can combine vitamin d with bisphosphonates. Or u can combine vitamin d with ca and bisphosphonates. However it is important to see ur budget and other things before finalizing anything else.
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