we did plasma measurement of cho and tri. in our mouse samples, now we would like to measure direct level in the fresh tissues, does anyone know where we could do this around montreal area, or any kits that can be used for this!
first make a homogenate of the tissue, perform an lipid extraction , B&D, resolve the lipid in triton 2% and use a kit (for example rouche , diasys) for TG and Cholesterol measurements
Along as I am working on an animal model study as C57lb/6J mice to determining the cholesterol concentration in blood serum and in the liver.
But now I am planning to extract the liver and the purpose wants to measure the cholesterol concentration. and if anybody know the procedure please recommend me or give me some idea about it.