Why does using lens (achromatic doublet lens) for imaging detection onto camera affect magnification in Interferometric Scattering (iSCAT) microscopy?

28 December 2020 2 8K Report

Hello every scientific explorers!!

Recently, I have been interested in Interferometric Scattering (iSCAT) microscopy, the scattering-based imaging microscopy.

One of the problems I am facing is the effective magnification of the image detected by the camera.

Jaime Ortega Arroyo; Daniel Cole; Philipp Kukura; Interferometric scattering microscopy and its combination with single-molecule fluorescence imaging. Nature Protocols. 2016, Vol.11, No.4
André Gemeinhardt; Matthew P. McDonald; Katharina König; Michael Aigner; Andreas Mackensen; Vahid Sandoghda; Label-free imaging of single proteins secreted from living cells via iSCAT microscopy. J.Vis. Exp. 2018, 141, e58486

In both papers, they use f = 1000 mm, f = 500 mm imaging lens with 100X 1.4NA objective in detection pathway and the effective magnification becomes 500X , 300X, respectively.

All I know for magnification is using two or more different focal length lenses.

Can you tell me why using just one lens can affects magnification of the image??

Thank you!

Radek Machan

Hi, well, as the description of the setup you provided says, they were actually using two lenses - the objective lens and the imaging (or tube) lens. The final magnification of the image depends on the combination of the objective lens and tube lens.

Jaesang Yu

I never thought about the focal length of the objective lens!!

Thanks for replying. It really helps me :)

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