I am working with some colleagues on historic FFPE sections from multiple sites that have been (unknowingly) cut onto different types of glass slide.
We have used Thermo SuperFrost Plus (J1800AMNZ) and Thermo Polysine coated (J2800AMNZ). Both are positively charged.
I know that positively charged and non-charged slides may display different staining properties; I wonder if anyone has any experience with different charged coatings and whether this is something we need to be mindful of regarding quantitative analysis?
Good day. Actually , positive charged and polylisine coated slides are used for immunohistochemistry because it prevents fall of section from slides during rinse in buffers heat antigen retrieval. It doesn't play role in quantitative analysis of results
Good day. Actually , positive charged and polylisine coated slides are used for immunohistochemistry because it prevents fall of section from slides during rinse in buffers heat antigen retrieval. It doesn't play role in quantitative analysis of results
The positively charged glass has nothing common with staining properties. It only prevents the fall of the material from slides when doing immunohistochemical reactions
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