I'm looking for a protocol for doing fluoresence microscopy to see the mitochondria shape/AR, on PBMCs from human blood?

More Linoy Israel's questions See All

How can we ensure clarity when discussing ML algorithms with non-technical individuals?

I have observed a notable trend wherein individuals from diverse fields are transitioning towards domains such as data science, data analytics, and machine learning (ML). Concurrently, there is a...

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How many ug/particles of EVs is needed for doing proteomics?

How many ug/particles of EVs is needed for doing proteomics? We are doing many steps for purify EVs of human plasma and at the end of the process we are getting just 1/2ug/ml concentration of...

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¿Cómo puedo acercarme de forma metodológica al impacto a largo plazo del maltrato en la salud mental y física de los niños maltratados ?

Con esta pregunta busco orientación sobre como abordar de manera sistemática y metodológica el estudio del impacto a largo plazo en la salud de los niños que han sido víctimas de maltrato. El...

10 March 2024 6,408 1 View

How to calculate the blank in BCA assay?

Hello, I have some problem when using RIPA buffer during reading BCA assay. I'm getting high value of the RIPA's absorbance so I'm getting negative values. I have Standard curve diluted with PBS...

14 February 2024 4,484 2 View

Exchange rate volatility and it's implications ?

research methods

14 February 2024 1,315 3 View

What are the answers to these burdens?

As Academic Researchers, can we rely on information ( Particularly paper references) provided by Ai as a true source even when can't verify it on Google? 2. How do we use Wikipedia references...

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Book proposal titled "Biotechnological Applications for Soil and Water Improvement and Conservation" . Are you interested in participate?

Hello esteemed colleagues and academics, I am thrilled to share some excellent news with you: our book proposal titled "Biotechnological Applications for Soil and Water Improvement and...

26 December 2023 877 0 View

What level of protection does the measles vaccine provide?

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Please help me with the following Yasara error?, it appears when I tried to do an homology of a protein.?

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09 March 2023 457 0 View

Any expert who has modeled soil compression in comsol, using a machine to make brick (adobes)?

Im really interesting in the stress faces of the compression machine to make bricks in order to optimize a brick machine, to be hable the manufacture of a machine accessible in rural areas of...

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How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

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Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

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Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

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Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Is there anyone with experience in TEM analysis who can assist with a manuscript for an upcoming journal?

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How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

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Why activated CAR-Jurkat cell could not kill targets?

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Why do we equate male and female arousal?

Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)

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Weak DAPI staining after immunohistochemistry - how to improve?

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How to isolate lymphocytes from mouse spleen?

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Irene Mariam Roy

Try Mitochondrial dyes like Mitotracker green which goes and binds to the total mitochondria of cells. It can be visualized in FITC range

Linoy Israel

Thank you! Do you know what is the best concentration for this type of cells?

Goodwin G. Jinesh

In addition to mitotracket, You can also try JC1 dye as separate batch, which gives polarizarion status.