I am using a toluidine blue O preparation for staining different parts of wheat plants to observe a fungus growing in them. This works really well for lighter tissues of the plant! However, the stain and chlorophyll interfere with each other and I cannot see inside the cells of the leaves. What is the simplest clearing method for leaf tissue you know of?
I do not have access to paraffin or a microtome.
Maria Schauer
Hey Rachel,
Easiest and cheapest method: Put your leaves in 70% Ethanol (or even 90%) for a longer time. That will remove the green colour.
What you could also try is a mix of Ethanol and Acetic Acid (9:1), that is way faster and also works as a fixative.
Cheers,
Maria
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