I'm tracking endosomes inside the cell to graphically show an anterograte (towards the membrane) or retrograde (to the perinuclear zone) traffic. What I am doing is tracking edosomes along their path in order to have the trajectories and differentiate the two types of transport with two different colors, using ImageJ. I know that with Imaris I can create a vector map of the different trajectories inside the cell but not knowing how to use Imaris, I was wondering if it was possible to do something like this with ImageJ (you can find a clear visual example of what I would like to achieve in the attached image "c"). Is there a plugin that does this? I can't find much on the internet.
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