Any suggestions regarding IFA in 96 well plates?

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In my CFD simulation analysis, I found high turbulence intensity with low indoor air velocity in a naturally ventilated room. Is it OK??

In my CFD simulation analysis, I found high turbulence intensity with low indoor air velocity in a naturally ventilated room. Is it ok??

25 January 2021 5,336 10 View

Are two text files obtained separately from qiime 2 can be used together to make diversity graphs?

I have two sets of 16s microbiome sequencing data. The animal model, facility, diet vendor are the same in both sets. The only change is in the treatment. For analysis, can I use both the...

29 December 2020 6,976 3 View

How to estimate water volume using remote sensing?

I want to estimate water volume from different water surfaces. I have used Landsat data before. Is there any method to extract water volume from NDWI or other Landsat bands? If not then, what are...

21 October 2020 4,775 11 View

Any data available on traffic distribution for underwater wireless communication/sensor networks?

I am looking for any source of information that describes how often an underwater wireless communication network is utilised on a certain day. For example, if you consider an underwater wireless...

07 October 2020 4,340 6 View

Can anyone endorse me for arXiv.org?

Hello, I need an endorsement to submit my preprints in arXiv.org. I am in the bioinformatics research field. The arXiv requires an endorsement for me because my institution doesn't provide any...

02 October 2020 1,041 4 View

Name of some mastermixes which contains UNG enzyme?

Hello, We are planning to work with HBV viral load. I have read several articles on HBV viral load quantification and came to know that they have used such mastermix that contains UNG enzyme to...

16 September 2020 7,911 2 View

Could you please recommend any good tutorials for natural ventilation in ansys with ref./pdf?

I am looking for a good tutorial for natural ventilation of a small room in Ansys fluent with ref/pdf for a detailed study.

22 June 2020 4,992 4 View

How to prepare 2x one step RT PCR mastermix?

Hello, I am working on developing a real time RT PCR based kit. For that, I need to prepare 2x one step RT PCR mastermix. I am using PCR Reagents and Verso cDNA synthesis kit from thermo...

03 May 2020 8,796 3 View

What kind of microstructure of Low Carbon Steel favors the maximum yield stress (>400 MPa)?

Micro-structural effect to Yield stress and yield ratio of low carbon steel.

13 March 2020 4,018 2 View

Why discontinuous yielding occurs?

The relation of discontinuous yielding with micro-structure of low plain Carbon steel

13 March 2020 527 4 View

Are ZnS nanoparticles moisture sensitive?

Hello Everyone, I tried to form ZnS nanoparticles using the hot injection method and was able to form the same (confirmed by XRD). When I centrifuged, after the reaction I got a kind of white...

17 February 2021 1,709 3 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

Pile-Raft connection?

Greetings, I was wondering what is the optimal way to connect the reinforcement at the top of the pile (the part that connects to the raft foundation): -The pile bars are left straight and...

16 February 2021 2,007 3 View

Best protocol for immunogold using araldite resin ?

Hello, We perfom Tokuyasu method (ultrathin cryo sectionning) very often to make immunogold experiments. This time, we need to make a PLT technique with K4M or LR white resin. Our results were...

14 February 2021 4,959 1 View

Resume Book UEP2013 Asian Edition, dis somebodey has it?

I am looking for a copy of the resume book of UEP2013 Asian Edition (Urban Environmental Pollution 2013 Asian Edition) held in Beijing. If somebody has it, please I would like to obtain an...

11 February 2021 2,247 2 View

Does anyone have a stack of images of action potentials or abnormal brain scans?

I need a stack of black and white .jpeg/.tiff/.jpg/.png images across time of either action potentials, neurons firing, or brain scans (comparing disease and normal brain, disease progression,...

09 February 2021 2,648 3 View

Regarding immunology, transmissivity, herd immunity and vaccines in Sars CoV-2?

This is a follow-up on an earlier question regarding mechanistics of vaccines: why current vaccines don't block transmission. In summary, they don't engage mucosal immune cells to produce IgA....

08 February 2021 8,321 3 View

ACE2 Blotted as a double band?

Hello, I have recently run a western of Vero cells for ACE2. I detected ACE2 at the correct size, but it presents as a double band. The sample was prepared by lysing cells in 1X Laemmli...

07 February 2021 6,973 3 View

SARS-CoV-2 Spike Protein Western Bands?

Hello, I am western blotting SARS-CoV-2 spike protein from lentiviral using an antibody against S1. To prepare samples, I mixed concentrated lentivirus 1:1 with 2X Laemmli buffer and boiled...

07 February 2021 3,643 4 View

Why do we wash azolectin with acetone?

I want to prepare liposomes made of azolectin (sometimes spelled as asolectin). I start with the azolectin powder, and many protocols say that I should wash it with acetone (dissolve, centrifuge,...

04 February 2021 2,738 3 View