Hi,
I am interested in a chromatin state over one of the promoters in different cell lines.
I would like to check it by ChIP-qPCR.
The question is how to show it? Is a one set of primers enouth and just do three repetitions? Or do you need more? If yes, should they be overlapping/close to each other or rather far away?
Also, how many chromatin markers do you need to check? Is just one fine?
Many thanks for any help!
Joshua Beytebiere
Are you looking for specific histone modifications? Such as H3K27ac? If so you do not need multiple primers sets unless you have a very large promoter. For humans most promoters are between 100bp-1000bp therefore if you are on the larger end designing a few primer sets could be useful.
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