Do you consider it is important to vindicate Spanish as language, in scientific issues and publication of scientific articles?

03 February 2021 3,756 4 View

Is it possible to get genomic context data on bulk of accessions?

I'm trying to build local gene synteny to identify homologs between different species. To do that, I've been accessing each individual gene of interest, going into gene view in NCBI and...

04 November 2020 9,038 2 View

No title

I was working on thermogravimetric analysis for some materials, however, because of the pandmy I stoped using the STA TG DTA-DSC 1600 for like 6 months, when I was able to work again with it, the...

11 October 2020 3,865 2 View

Can anyone give me a guide regarding vcf files?

I have a VCF file with SNPs using the IGV program, when uploading the file some SNPs that are observed in a particular gene in IGV, when verifying it in GDV or Ensembl do not match

10 August 2020 699 3 View

Can 6M Urea inhibit lysozyme action? or this happens the other way around?

I mix both components together with other substances to treat my bacterial pellet and extract protein, but I don't know if Urea 6 M or lysozyme loses its effect

24 April 2020 4,098 14 View

Can someone please share a protocol for the production of recombinant proteins in E.coli BL21 DE3? or the use of plasmid pET 23B?

this for obtein a protein of plasmodium please

24 April 2020 4,302 3 View

How to download citation network data from Web of Science (WoS)?

I want to do citation analysis on papers on ecnomics, but I don't know exactly how to get this data from Web of Science, though many sources say this is one of the very good datasets in this area....

16 March 2020 4,184 4 View

What is a good dataset and technique for citation analysis in Economics?

I would like to track the presence of some ideas in papers in economics, and I have been reading about citation network analysis and I have found some datasets (like the...

26 February 2020 2,848 1 View

Can I use an antibody with application for FC, to do IP or WB??

Hi there! I need to do immunoprecipitation and Western blot to obtain and detect a protein and I already have conjugated antibodies with FITC and PE for flow cytometry. I was wondering if there...

17 February 2020 223 3 View

Can someone explain about Different output of C/T vs A/G CSN1S2?

A NGS panel of truseq was performed, the SNP expected of CSN1S2, Ch 6, Position 87266177 are C/T, but we received A/G. There is an explain about that?

28 January 2020 1,118 2 View

What is the difference between genotype and haplotype data in the ped format?

Hi everybody In the ped format for genotype, alleles of any SNP are represented by two columns (one for each allele, separated by a space). Is a column sufficient for the haplotype to...

27 February 2021 1,965 1 View

Why the sum of the observed homozygosity and observed heterozygosity is different from one?

I had computed the HWE test using the --hardy command of the plink 1.9 program, to find the observed and expected heterozygosity. The observed and expected homozygosity has been calculated using...

24 February 2021 5,252 2 View

How to convert .txt into plink files (ped/map or bed/bim/fam)?

I have a huge oncoarray_genotype_XXXX.txt file with 11.7 GB size. I would like to convert this file into adequate files for analyzing in plink software. However, I could not find how to conduct...

08 July 2020 9,302 2 View

How can I merge two PED/MAP file sets from different chromosome builds for GWAS study in PLINK?

Hi, I have two data sets from the illumina omniexpress snp array platform. The first data set was mapped using the GRCh37 build and the second one was more recently read using the GRCh38 build....

14 June 2020 6,281 1 View

Can someone please help with inputting PLINK assoc.logistic output files into METAL to perform a meta-analysis?

I need to perform a meta-analysis of 2 GWAS studies and I need assistance with the input study files (plink --assoc.logistic) as well as the output meta-analysis file (.TBL). - Firstly, I am not...

07 June 2020 5,831 1 View

VCF File SNP Analysis ??

Hello all, I have 60 samples of Whole Genome Sequence (BAM, VCF ) File. Now I want to do SNP analysis ( I am trying to find some positions which differ among case and Control ). I did with...

30 May 2020 980 4 View

Should I test for HWE in dataset of inbred animals without random mating?

Hi, I am doing a GWAS analysis of european bison's a dog's SNPs. Right now I am doing multiple data clean up, according to previous studies and what I have learned. I was thinking, if it is ok to...

18 March 2020 3,103 3 View

What mistake in GAPIT could lead to high FDR adjusted P-values?

Can anyone suggest a reason why the association between my SNPs (90 thousand) and two quantitative traits (BMI and age) has a FDR-adjusted P-value over 0.9 for all my SNPs? I think it is very...

18 March 2020 8,080 3 View

Is it correct to use Genomic Control (GC) adjustment in association analysis of candidate SNPs?

Hi all, I have a list of

07 August 2019 7,158 3 View

No title

20 July 2019 4,549 3 View