Something about our protocol or reagents is wrong because we couldn't manage to succeed.
I am investigating a topic about Native Speaker Pronunciation. I am looking for any resources related to this topic. I am curious whether students should still be motivated to obtain it or perhaps...
02 March 2024 7,375 10 View
I am investigating career shocks, and using Seibert et al. (2013, 2016) scale. However, these account for shocks that happen within the organizational setting, and I am also interested in...
06 December 2023 4,890 0 View
Hi all, I have just prepared CORT:HBC (sigma) in water, and I did not used all the solution, do you know if it can be stored once is reconstituted, and for how long? Thanks in advance, Renata
05 November 2023 5,543 0 View
I would like to isolate exosome from biobank of frozen blood and lung tissue. The storage of the samples reduce the possibility to isolate exosomes?
19 March 2023 4,088 0 View
I performed exosome isolation from cell culture media by using a kit for cell culture media. Now, I would like to perform the isolation of exosomes inside the cells. Which protocol can I follow?
14 March 2023 4,595 4 View
I would like to analyze the expression of immune markers on exosome inside macrophages by using immunofluorescence staining. This is my first approach in the field of exosomes.
09 March 2023 7,148 0 View
I am looking for a software/online tool that will help to identify possible sites prone to post-translational modifications (including deamidation, isomerization, protease cleavage, oxidation...
10 May 2022 9,206 2 View
I am a beginner in Geometric Morphometrics and I am working with archaeological artefacts. I have these 3 doubts in regards to semilandmark building on TPS: 1. before placing the LM curve in all...
23 March 2022 1,100 8 View
Dear researchers, I'm working with doxycycline and β-cyclodextrin. How can I be sure that drug is small enough to fit inside the cyclodextrin cavity? I found that the β-CD cavity has diameter...
17 October 2021 9,239 2 View
So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...
02 March 2021 1,836 2 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
Hi RG family. My team and I are working on some SCOPUS publications and we need co-authors who are willing and capable of undertaking both qualitative and quantitative-based studies. The scope...
02 August 2024 7,843 0 View
Hi RG family. My team and I are working on some SCOPUS publications and we need co-authors who are willing and capable of undertaking both qualitative and quantitative-based studies. The scope of...
02 August 2024 8,572 0 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
Hello Everyone, While doing Boehm titration, We use nahco3 in order to determine carboxylic acids. Then the mixture was agitated. After a while, the solution was filtred and HCl was added. After a...
29 July 2024 4,048 4 View
Hello everyone, I am currently looking for tools to recovery viral genomes from bacterial genomes, not metagenomes. However, I have only found tools that are designed for retrieving and studying...
28 July 2024 8,953 1 View
I've attempted to use this method to synthesize copper nanoparticles. Copper nanoparticles can be synthesised using a variety of precursor materials. CuSO4, distilled water, NaOH, and EDTA are...
28 July 2024 8,027 3 View
Hello, I would like to ask which is the best terminology to use for describing a negative control in an experiment (drug testing in-vitro, viral infection): NC (negative control) or NTC? Thank you...
18 July 2024 4,183 2 View
Sulfuric acid has changed color to yellow.
16 July 2024 6,964 0 View
I have a small molecule reagent with a boc-protected amino group. Now the reaction needs to be reacted at 37°C for 30 h. Is this protection group stable?
12 July 2024 3,745 2 View