Is ANSYS Fluent good for simulation of a three phase reactor(stusy of its hydrodynamics or mass transfer...)?

Hello everybody, I would like to simulate a three phase reactor and study its hydrodynamics. I would like to know which type of software is better? I have a good command of ANSYS Fluent and I am...

10 February 2021 4,303 3 View

Could anybody give me information about room temperature degradation of silicone oil to silicon oxide?

Does anyone have any experience of room temperature degradation of silicone oil to silica in presence of acids?

26 December 2020 1,275 4 View

Should i add ATG in the beginning of my polypetide sequence when i want to insert it in a phagemid?

Hi I want to make a recombinant m13 phage displaying my peptide as a fusion peptide on its surface pVIII. Should i add ATG at the beginning when i want to insert it in phagmid vector? Previously,...

18 December 2020 9,931 2 View

How can I download Haiti earthquake datasets?

How can I download Haiti 2010 GeoEye Satellite images or any other datasets regarding this mentioned study area? Is there any FTP server or website to access? Or If anyone has this data set, I'm...

07 August 2020 4,872 3 View

What is the most appropriate qualitative analysis method for my research on representation of dreams in the works of Medieval Sufi?

Hello to all, I am doing research on the representation of dreams in the works of Muslim Mystics in the middle ages. I am interested to know what "dreams" meant to them and how they used it. I...

06 June 2020 7,205 4 View

What are the best python libraries to view 3D point cloud?

I wanna view a point cloud in a 3D visualization and try to rotate the 3D plot to see what's happened to the point cloud? which python libraries would be applicable to handle such this? Any...

18 March 2020 6,804 3 View

Which family name is correct for the insect species listed in the table below?

Hi friends The following table shows insect species from 3 orders. In conflicting sources, they are introduced from different families (family 1, family 2 and family 3). What do you think about...

02 March 2020 5,140 3 View

Atomtype NPri not found hn gromacs?

I tried to make .tpr file in gromacs and there is a fatal error: Atomtype NPri not found hn gromacs. How can I solve this problem with an easy way?

29 December 2019 5,081 3 View

Please the scientific name of this insect?

Hi This species was collected from northern Iran. Yours sincerely Salehi

11 July 2019 1,460 22 View

What is the relation between the force composite should withstand and the percent of glass fiber in it?

Dear Friends, I need to know how can I estimate the amount of glass fiber in composite produced by poltrusion, based on the force that final product is supposed to withstand?

11 March 2019 467 4 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

What is the best concentration of cDNA to be used for qPCR?

I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...

24 February 2021 8,618 3 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

RNA degraded after good nanodrop readings?

23 February 2021 8,850 2 View

Is there a way to extract total RNA and protein from cell culture at the same time, without denaturing protein?

Hi all, Is there a way to extract RNA and protein from the same cell culture sample? Because we need to use the protein for gelatin zymography at later time, we are looking for a method that can...

22 February 2021 5,341 5 View