Microbiology is a broad term which includes virology, mycology, parasitology, bacteriology, immunology and other branches. | Contact experts in Microbiology to get answers
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Questions related to Microbiology
I am following the protocol set by Kariluoto & Piironen (2009) Total folate , and towards the end of the experiment the volume taken for final estimation in each well would be 400 uL (100 uL...
10 October 2019 6,667 5 View
I am using specific probes for detecting and quantify bacteria with FACS, however in spite, it is supposed to work (Previous studies and In silico analysis of the probes), I only get high...
10 October 2019 9,930 2 View
Hi everyone, I'll start expressing a human protein in the yeast Pichia pastoris. In our research center all the flasks, graduated tubes and similar materials are cleaned and sterilized by...
10 October 2019 2,479 3 View
Can I make 100 ml of Müller-Hinton agar from 38 g mixed in 1 L Müller-Hinton agar solution?
10 October 2019 6,086 7 View
I am interested to make a phylogentic tree as a requirment to prove my putative novel bacterial species. I would like to know if I should only take the Type-strain 16S RNA sequence of my related...
25 September 2019 5,678 11 View
I want to measure BCA Protein for normalization purposes. Let's say I have two flask. With cells irradiatied with a big dose (>10 Gy) and non-irradiated cells. Number of cells in non-irradiated...
09 September 2019 323 3 View
I have been working with spirit blue agar now for several months, and I'm having a hard time finding a conclusive answer to tell me if I have had a positive result for a lipase. Some published and...
09 September 2019 429 4 View
I have a question about gellan gum (aka Phytagel, Gelrite). Basically, why is it mostly used as a gelling medium for plant tissue culture and not so much in culturing other organisms? In my...
09 September 2019 5,415 4 View
I know this is a very broad question, but in your experience how sensitive is qPCR? It is reported that qPCR can detect even 1000 CFU/ml or less of bacterial species in human samples, and...
09 September 2019 4,520 5 View
09 September 2019 4,858 12 View
This was isolated from a 1000 year old Quercus robur oak tissue culture. The colonies on LB low salt agar plates look glossy, white-ish, smooth edges. They grow very slowly. In a month at room...
09 September 2019 4,076 8 View
Hi ..., it would be really nice if someone has original research data on the Kovats Retention index of 4-Methyl-2-oxovaleric acid. I already checked NIST Chemical Webbook, but chromatographic data...
09 September 2019 9,183 3 View
02 September 2019 10,041 3 View
i could like to know if there are any international standards for a microbiology laboratory for production of bio pesticides.
26 August 2019 5,017 3 View
I have to do a competition growth curve with two different isolates (Salmonella and Lactobacillus). What approach would you use to do this? I thought I could grow them seperately (Salmonella in...
08 August 2019 348 3 View
Can you please recommend journals in the fields of Microbiology, pharmaceutical sciences, natural products which are indexed in Scopus till now (2019) ,and with publication fee less than 200$...
08 August 2019 6,572 4 View
08 August 2019 6,890 3 View
At our lab, we frequently grow Staphylococcus aureus using TSB or Chemically Defined Media. The latter gives us some control on the conditions we want to impose to the organism, but I was...
08 August 2019 1,222 6 View
I need to know the CFU / ml of staphylococcus aureus. For this I would like to know if there is any investigation that determined the CFU / ml at a given absorbency at 625 nm. Thank you.
08 August 2019 9,506 4 View
The topics concerning public health based will be effective in my view but there is no compulsion they can be of medical based too.
08 August 2019 2,912 17 View
Which is a better medium for preparing 0.5 McFarland turbidity standard for antibacterial activity assay? saline or Müller-Hinton broth?
08 August 2019 6,026 21 View
06 August 2019 1,916 5 View
I made some blood agar plates with TSA+5% blood. They turn into chocolate color after 48hr in 37℃, but the commercial one doesn't. How can I make the same stable blood agar plate? Add: I use...
06 August 2019 6,915 4 View
I am analyzing the safety aspects of two food production sites producing similar products. I wish to compare these geographically distinct sites across seasons as well as processing steps. I have...
16 July 2019 640 3 View