Microbiology is a broad term which includes virology, mycology, parasitology, bacteriology, immunology and other branches. | Contact experts in Microbiology to get answers
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Questions related to Microbiology
I am currently reporting results from a hierarchical multiple regression analysis. I'm not sure how to report the significance values as many papers report different things. I believe you should...
13 April 2021 834 14 View
We have lost many flasks due to contamination to B. cepacia. We have added Pen-Strep to no avail. What are other antibiotics that could be used in culture media that does not belong to...
29 March 2021 8,225 8 View
I would like to know what is the best dose of levan to use in vivo and the treatment period. I am using mice and I would like to gavage them with levans.
25 March 2021 8,057 4 View
I want to know about the different media used to check the growth and characterization of cadmium resistant bacteria to know the best media for its growth and also to aid identification of the...
23 March 2021 900 6 View
I am performing wastewater treatment thought different microorganisms such as microalgae and afterwards determining the lipid content in the biomass though Bligh and dyer method. Due to the COVID...
11 March 2021 5,007 3 View
I used 100mg in 100ml to prepare glucose standard curve so as to have concentration in mg per ml. It was incubated for 10 mins. I have been able to use the graph equation to calculate enzyme...
07 March 2021 7,258 2 View
Hello Everyone, The past few years have seen a lot of new cyanobacterial taxa being described using a polyphasic approach. It will be interesting to know that what are the various good things...
06 March 2021 4,307 4 View
Hi everyone, I am a new PhD student with very little microbiology experience and I have a few questions regarding the growth of Lactobacillus Reuteri which I hope you can help me with. I want...
04 March 2021 9,855 3 View
Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...
25 February 2021 3,478 6 View
I am aware of a study that has compared the use of citrated and defibrinated sheep's blood in microbiological media (Hair Sheep Blood, Citrated or Defibrinated, Fulfills All Req... ), but can...
11 February 2021 6,167 1 View
How can I know the stationary phase of growth of several bacteria? We are aiming to collect exoproteome of several bacteria. We know that the ideal time to collect exoproteome is during the...
01 February 2021 4,775 10 View
I have tried to use TTC agar overlay method for a few times to identify respiratory-deficient yeast which were affected by EthBr. The problem is that cells which should have stayed white also have...
23 January 2021 731 10 View
Need to check quorum sensing activity of Vibrio campbellii.
21 January 2021 748 5 View
I have an unstained section in the PEN membrane slide already and need to stain it with Cresyl Violet Stain to isolate RNA/DNA, do I directly put the solution on the section or do I dehydrate...
07 January 2021 706 0 View
I received several strains of bacteria from another lab far away. However, one of the samples appears to have high fungal contamination. When I grow it in broth, I see lots of fungal...
04 January 2021 2,946 16 View
Hi, I'm expressing and purifying a membrane protein which at the C-terminus has a short linker followed by a TEV-mEmerald-TwinStrep tag. I can solubilise and purify the protein with...
02 January 2021 2,406 7 View
the study of microorganisms is called microbiology Microbiology is the study of microscopic organisms, such as bacteria, viruses, archaea, fungi and protozoa. This discipline includes fundamental...
14 December 2020 8,476 6 View
We want to screen halotolerant protease producing bacteria. Since Skim milk (HiMedia) is available in our lab, therefore, we want to screen extracellular protease by this compound/ supplement. We...
05 December 2020 7,803 6 View
Microbiologist’s opinion needed Hello everyone, Short version: We want to study microbiologically influenced corrosion in simulated seawater. How do we cultivate marine bacteria in the...
03 December 2020 6,913 3 View
How long is it possible to store the bacterial culture at 4C?
03 December 2020 2,891 11 View
I've been trying to recover some bacterial cells by streaking them on agar plates, without success. The stock has been kept in good conditions. But, there is a white pellet at the end of the...
25 November 2020 5,705 8 View
For Fungal Spore suspension, can I use Tween 20 instead of Tween 80 as both are detergents?
20 November 2020 9,460 2 View
I found this organism in my Petri dish following soil serial dilution. It is embedded in the media and is showing a branched- like pattern. I am relatively new to microbiology and I can not figure...
05 November 2020 1,908 11 View
I have prepared PBS buffer and components for M9 medium and I store it in freeze, I do not use it if it is more than 2 weeks old. From time to time I check the sterility of these solutions and...
05 November 2020 9,631 6 View