Microbiology is a broad term which includes virology, mycology, parasitology, bacteriology, immunology and other branches. | Contact experts in Microbiology to get answers
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Questions related to Microbiology
control on microorganisms
21 November 2024 1,286 1 View
Good afternoon: I need a recommendation from someone who is preparing a CBB G-250 solution in MIlli Q-water, which can be used as a dye in protein hydrophobicity determination. We have an issue...
20 November 2024 5,095 3 View
I am performing an RNA extraction on intestinal epithelial organoids using Trizol LS. After harvesting, homogenizing, and adding chloroform, I centrifuged the samples to separate out the phases....
15 November 2024 906 4 View
If there is a research model with one IV, one DV and one continuous moderator. So, how to check the parametric assumptions in SPSS? Include an Interaction Term: Create an interaction term by...
05 November 2024 1,054 2 View
the closer the sample is to a ladder, the more curved line i get. I run the samples in 0.7% agarose gel for 30 minutes, 150mV. Why does it happen and how can i improve my gel?
04 November 2024 5,050 4 View
I'm working on the method development for the analysis of various PPCPs and emerging contaminants in aquatic environments, currently focusing on SPE recovery by spiking compounds in Milli-Q water....
30 October 2024 3,010 7 View
What changes have taken place in mitochondria in this electron microscope picture? Is there autophagy or iron death, or anything else?
25 October 2024 587 5 View
Hy, in my research I need to extract RNA from mice gut samples for further cDNA and qPCR. However, the results are really inconsistent, sometimes it's 700ng/µl, and for another replicate which has...
24 October 2024 1,174 1 View
I would like to reuse my primary antibody for western blotting. I am looking for an optimal way to store. I block my membranes in 5% skim milk followed by washing and primary antibody incubation....
23 October 2024 9,689 3 View
I want to deliver docetaxel and miRNA by a niosome to cell line. regarding to docetaxel which fluorescent dye is better to use? CY5 OR FAM Thank in advance for your considering and response
22 October 2024 2,330 2 View
Hello I am having a trouble with the result of agarose gel electrophoresis of the PCR product of Entamoeba gingivalis DNA Samples: Paper point samples from the sulcus(between tooth &...
14 October 2024 6,050 10 View
I want to infect IAV in A549 cell line. It is usually recommended to use TPCK along with it. I was curious to know what is its role in IAV infection.
27 September 2024 326 1 View
mecky@DESKTOP-P2C3VM2:/mnt/c/cta-ns2$ python3 cgenff_charmm2gmx.py UNK unk_fix.mol2 unk.str charmm36-jul2022.ff Traceback (most recent call last): File "/mnt/c/epigall-ns2/cgenff_charmm2gmx.py",...
20 September 2024 8,939 4 View
Get a life, Mind your OWN BUSINESS. HOW ABOUT WORKING FOR YOUR OWN SUCCESS, RATHER THAN RESENTING OTHER'S SUCCESS. PUT IN THE HARD WORK TO DEVELOP YOUR OWN MASTERY. STOP BEING ENTITLED WHEN YOU...
05 September 2024 9,555 17 View
I want to construct a phylogenetic tree based on the STs of K. pneumoniae. Seven housekeeping genes (gapA, infB, mdh, pgi, phoE, rpoB, and tonB) were sequenced using Sanger sequencing, and the...
12 August 2024 6,924 1 View
I am a PhD student, currently studying yeast endobacteria. The endobacteria our group work with are however cultivable although their genome sizes are significantly cut to half. The genome...
07 August 2024 7,440 2 View
I suspect that the endogeneity is caused by a missing variable and simultaneity with the mediator influencing the DV and the DV influencing the Mediator . What is the procedure using SEM to solve...
09 July 2024 8,717 0 View
H index in research gate
01 July 2024 7,757 8 View
Hello, I have a couple of questions regarding small RNA sequencing for miRNAs if anyone has done it: 1. Would a regular miRNA isolation kit from Qiagen be suitable for this purpose? 2. Would the...
26 June 2024 1,349 3 View
Considering the manufacturer did not supply it, I need to equilibrate the column for my western blot experiment.
25 June 2024 637 1 View
I only obtain DNA smears from apoptosis induced cells, and no characterisitic DNA ladders/fragmentation Inducing apoptosis in Jurkat cells as follows Per 1 million cells in a well, final...
24 June 2024 3,884 0 View
I am trying to isolate RNA from Mouse Ovaries by using Qiazol. But I am not getting good results. After completing all the steps when I measured the RNA by nano-dropping, I saw there are very low...
21 June 2024 8,614 10 View
So in my Project I am working on Compounds that have chelator characteristics, as they bind different transition metals, with different stoichiomoetries. I wanted to determine the binding...
11 June 2024 6,445 1 View
Warner used to sell 8161 glass, but Harvard Biosciences bought Warner and doesn’t appear to stock it anymore
05 June 2024 5,777 2 View