Microbiology is a broad term which includes virology, mycology, parasitology, bacteriology, immunology and other branches. | Contact experts in Microbiology to get answers
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Questions related to Microbiology
I know many websites have simple tools like transcription and translation available, but are there any analysis tools that researchers need that either do not exist or are not publicly available?...
29 July 2023 8,635 3 View
How do scientists use metagenomics and high-throughput sequencing technologies to study microbial communities and uncover novel microbial species and functions?
15 July 2023 8,462 1 View
I am running ELISAs. I run blank samples, without standard, and I subtract the mean OD value from the blank wells from the ODs of the other wells (standards and samples). Nevertheless, I have...
13 July 2023 9,167 3 View
I am trying to design a primer for a specific gene and the Tm is coming near to 60 degrees when I select a minimum of 30-35 bp, for forward as well as for reverse primer. As the template is too...
07 July 2023 4,731 4 View
Hello scientific community. I wonder if someone who is experienced in high quality drawings can share me on how to draw publication quality figures in general like path diagram in structural...
13 June 2023 9,130 1 View
Give me technical guide on decreasing salinity level in Larval rearing tank. I want to drop down salinity level from 25 PPT to 0 ppt in 7 days duration in L.Vannamei Laraval's PL stage in larval...
06 June 2023 2,153 3 View
Hello all, I recently started to use peptides for cellular studies. Unfortunately, when I was designing the peptides I did not have a FITC tag to them (the peptide was synthesized by an external...
01 June 2023 1,620 3 View
My question is for phytoplankton expert, if i found some phytoplankton which are new or rare to that habitat, where i can verify and publish it ?
26 May 2023 6,102 0 View
Why do we do MIC, can disc diffusion assays be enough to check the antibiotic resistance pattern of a bacteria?
24 May 2023 7,119 2 View
I need to prove the hypothesis
16 May 2023 9,557 2 View
Isolated from a waste water with high organic pollution with a length of 13-64um and width of 2-6um. SEM snippets is attached below with a scale of 1 um.
03 May 2023 4,419 0 View
Hello, End of last year I have developped an indirect ELISA method to detect a couple of caseins in fermentation supernatants. It worked great until a couple of weeks ago. Since then, for each...
27 April 2023 9,449 2 View
I have RNAseq data made with selection by PolyA and other RNA-seq Total data. I want to join this data to increase the sampling within some subtypes I have few samples. How can I normalize these...
10 April 2023 4,879 2 View
I want to do liminology of my fish pond. My major concern is with different types of algae present in the pond. ....kindly share your experience regarding sampling of algae and cell count per...
05 April 2023 9,876 1 View
biofilm and quorum sensing genes of E.coli, not used drug but chemical material (Thiophene)
04 April 2023 805 11 View
I recently met an issue when GFP wasn't expressing in OE plants.. I want to know is there any method to analyze before agrobacterium transformation in Arabidopsis, whether my construct is working...
27 March 2023 3,925 3 View
Dear all, I am currently trying to determine the number of archaea present in an ecosystem with the 16S rRNA gene. I am normalizing the mcrA gene with the total archaea 16S rRNA gene. The mcrA...
17 March 2023 1,550 4 View
I am currently working on the synthesis of chitosan-copper nanocomposite for environmental remediation. I attempted to disperse the nanocomposite in distilled water and some buffers; while the...
13 March 2023 8,043 3 View
I am using Roche Lightcycler 96 machine to perform protein thermal shift assays. I added 15 uL of 50 uM protein and 5 uL of SYPRO ORANGE fluorescent dye in DMSO. The temperature ranged from 30 °C...
13 March 2023 1,179 1 View
I got an invite to join SciPinion and I can't tell if it is a predatory journal or not. Anybody have experience with them? The email opened with this: "Your peers have identified you as an expert...
15 February 2023 5,165 6 View
Prodigiosin extracted from Serratia, Boiling point- 554.3 Celcius, MW- 323.4 Da
11 February 2023 1,453 2 View
We are working on Biofilm and Quorum sensing genes of E.coli, we suggest some of reasons maybe:- Contamination, Annealing Temperature, Melting Temperature, DNA Extraction mistakes in the...
10 February 2023 6,939 10 View
What is the best method for DNA isolation from fish samples? Any experience and/or tips how to improve yield of genomic DNA from fish samples? What tissue is the best source? How much DNA is...
01 February 2023 5,703 1 View
I have a protein sequence with two cysteine residues and I would like to predict if those cysteins will form disulfide bonds. I am looking for user-friendly tools to do this, either online tools...
19 January 2023 1,476 4 View