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Questions related to Microbiology
During Glycine betaine estimation, the periodide crystals are to be dissolved in 1,2-dichloroethane. However, My current lab do not have a fume hood, therefore, I am looking for a safer, but...
4 weeks ago 6,453 0 View
dental materials
4 weeks ago 8,421 1 View
I overexpressed a FLAG-tagged protein in HEK293T cells, performed co-IP with anti-FLAG M2 beads, and attempted elution with 3×FLAG peptide, but the yield was very low. I suspect oligomerization...
4 weeks ago 6,431 1 View
Diagnosis of pathogenic bacteria
4 weeks ago 1,554 0 View
pathogenic bacteria
4 weeks ago 865 0 View
Hello, I am trying to use transverse cryosections of larval zebrafish tails for ISH and histological staining, but my sections often all off my slide (occasionally, one or two stay on). How I...
last month 3,147 2 View
ICZN Article 75.1. Definition: A neotype is the name-bearing type of a nominal species-group taxon designated under conditions specified in this Article when no name-bearing type specimen (i.e....
last month 6,422 6 View
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26 July 2025 7,767 1 View
initial Ni percentage = 1,84% (by XRF) Ni content in solution: 4791,9 ppm (by AAS) volume of leaching reagen: 1500ml Ore sample weight = 150 gram
19 July 2025 7,744 3 View
We are testing several chemical compounds on tumor cells, and after determining cell viability using the Resazurin assay (alamar blue assay), we find that the fluorescence results do not...
21 June 2025 6,159 2 View
I have been trying to standardize comet assay for sometime now but I just have not been able to see the comets whatsoever even in my positive control which are cells treated with hydrogen...
12 June 2025 8,666 0 View
I worked on a paper called "Exploration of Neuroscience Emerging therapies of hemangioblastomas," but I was not given credit as one of the authors because I did not have a Research Gate account at...
26 May 2025 4,763 1 View
Hello colleagues, I’m a PhD student in Hydrogeology at Shahid Beheshti University (Iran), and I’m currently working on my dissertation which focuses on optimizing groundwater exploitation to...
23 May 2025 6,428 0 View
The standards set by both the Trends in International Mathematics and Science Study (TIMSS) and the Programme for International Student Assessment (PISA), which are based on the National Council...
18 May 2025 1,364 0 View
How to embed sustainability into the curricula?
16 May 2025 3,179 11 View
I performed a DPM simulation in Fluent, but how can I calculate the DPM concentration within a specific region?
13 May 2025 5,606 1 View
I am having some trouble in the purification step of carbon quantum dots, I got the right dialysis bag for the process, but before the dialysis I would like to perform a centrifugation. The CQD...
11 May 2025 4,059 3 View
I'm wondering how stable a master mix of SDS-PAGE ingredients (without APS and TEMED) would be when stored in a 4ºC laboratory refrigerator. The master mix would contain water, acrylamide, Tris...
11 May 2025 8,161 3 View
In academic systems, credit is predominantly tied to authorship and publication. The individual who formally describes a new species becomes permanently associated with it, as their name is linked...
08 May 2025 9,054 1 View
treatment of oral fungal infections
28 April 2025 5,280 0 View
realistic/unrealistic doses
16 April 2025 6,351 2 View
Magnesium and calcium have high solubility in water, so they do not precipitate even when combined with chloride ions. Are there any other substances that can be removed in the form of precipitation?
28 March 2025 6,010 2 View
Hello Everyone, I'm conducting a study of cholesterol assimilation of bacteria in MRS (de Man, Rogosa, Sharpe) broth media , I need to perform o-phthalaldehyde method for cholesterol...
22 March 2025 8,976 0 View
I have to perform transformation of a vector into clinical isolates of Klebsiella pneumoniae. My vector consists of chloramphenicol resistance gene but the isolates are resistant to...
21 March 2025 4,265 3 View