I have a culture of heterogeneous bacteria that will NOT form endospores.
This bacteria was originally isolated in nature and is a complex culture of multiple species.
I want to be able to preserve samples of these cultures for future study, but I cannot get them to last more than a few weeks in a refrigerator.
I CANNOT freeze this bacteria.
Is there a way I can put this bacteria in a quiescent state that will preserve it for a few months in a refrigerator?
Thanks!
Chuck
Lyophilisation but with the use of a cryoprotectant (such as DMSO/ glycerol) , although not recommended to all. Being heterogenic, it will be quite a challenge.
https://bitesizebio.com/45159/how-to-preserve-microorganisms/
old paper that could help, states that calcium carbonate can help preserve non-sporulating bacteria for over 1 year:
https://onlinelibrary.wiley.com/doi/pdf/10.1002/j.2050-0416.1924.tb02648.x
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