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Questions related to Microbiology
My blood agar plate turns to chocolate agar plate after being incubated at 37 degree Celsius for 24 hours. I cooled down the NA agar to 50 degree before adding the defibrinated sheep blood. What...
18 January 2023 8,439 0 View
We are utilizing an aluminum 96well plate system with glass vials due to organic we are utilizing. We need to heat the system to 100C and not let any organics escape. We are having issues with...
10 January 2023 3,004 1 View
Why primer binding template instead of the two strands bind each other? The Tm of one strand is higher than primers. Complex structure of the template? The amount of primer is much higher than...
29 November 2022 5,804 1 View
Hi everyone, I am trying to grow marine cyanobacteria from my samples using BG11 and L1 as nutrient and agar as gelling agent. I found agarolytic bacteria thrives in my plates, liquifying my...
28 November 2022 5,392 3 View
Hi, i have now a fully working panel with lots of markers and will proceed to stain and compare samples, pmbc healthy vs non using flow cytometry. Which one of these programs to choose for...
23 November 2022 5,959 4 View
I tried to tosylation glycerol carbonate with sodium hydride base in THF solvent. but C=O bond in glycerol carbonate was broken. can you help me what is the reason of that? is it becauce of the...
15 November 2022 1,884 4 View
although we have already used f8 and r1510 primers but the results are not enough good
14 November 2022 6,918 0 View
I am trying to run an RDA or CCA (Redundancy Analysis, And Canonical Correlation) to illustrate the relationship between measured gene abundance and a set of env. variables. The environmental...
01 November 2022 8,309 4 View
The objective is to sequence two alleles, and PCR products are amplified in the same PCR, but better results are generally obtained for the shortest PCR product. How can I optimize reaction...
19 October 2022 6,080 4 View
I am preparing gastrointestinal fluids to test yeast survivability in-vitro. The protocol requires me to prepare two solutions, gastric juice and intestinal fluid, requiring me to add pepsin and...
18 October 2022 8,740 5 View
Hello, I've been tasked with validating a 2kb region of DNA. A colleague designed primers with the F melting at 53.8 and the reverse at 59.7. I believe they used 54C which had multiple bands show...
17 October 2022 5,448 6 View
How to set the protocol and how to set all parameters for the experiment?
13 October 2022 8,419 3 View
We intend to perform a Galleria mellonella infection assay, infecting them with clinical strains of Klebsiella pneumoniae, then treating them with two antimicrobials sequentially applied. Have you...
28 September 2022 8,616 1 View
I am working with MDA-MB-231 cell line. I am not looking for cancer protein or receptors. I would like to know specific mammary epithelial extracellular or intracellular species, that can prove...
15 September 2022 6,001 0 View
Nothing
27 August 2022 6,133 0 View
I am working with macrophages and want to optimize infection of macrophages with fast growing Pseudomonas and slow growing non-TB causing Mycobacterium abscessus (one bacteria at a time, not...
02 August 2022 3,474 2 View
Since, HepG2 is a non-tumorigenic cell line, if i inject it into a nude mice will the cancer be induced by the presense of it?
13 July 2022 7,479 1 View
Dear all, I have to draw the complete pedigree of a high number (>40) of dogs in a study regarding genetic diseases, so I started drawing the pedigree using the R package kinship2. The...
30 June 2022 302 3 View
We are looking for explanations of the differences between buffaloes and cattle, we have obserced low expression of FSHr transcripts between cattle and buffaloes, but we don´t have data to compare
27 June 2022 9,037 5 View
hey, i isolated from kefir drink .. after purification i tried to recognize the colonies under microscope but i found different colony ! any idea about it ?
21 June 2022 7,273 1 View
Hi everyone ,does any one know how can I make a positive control for autophagy pathway with NH4Cl? I’m working on nalm6 cell line
29 May 2022 9,791 0 View
Normally F1 plants suppose to be uniform both genetically and phenotypically and start segregating at F2, but I notice some differences in seed germination and seedling vigor (all things being...
25 May 2022 9,881 3 View
I am trying to bind lipid extractions as well as purified single lipids from mycobacteria to Nunc PolySorp 96 well plates to perform ELISA's. Lipids are insoluble in water, so when I try and...
25 April 2022 7,576 4 View
To give context, I'm trying to keep the cells in an anaerobic environment by sealing the plate with mineral oil and transparent 96 well plate seals. But, the bacteria don't grow once they're out...
24 April 2022 6,749 0 View