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Questions related to Microbiology
As a part of my dissertation I am subjecting colonies of E. coli to varying pH levels using hydrochloric acid. The colonies are grown over cell growth quantifiers and six hours into growth, when...
19 March 2020 9,148 3 View
Dear researchers, I am working on acidophilic bacteria and mineral interactions. I will use rock grains in my microbiological flasks and see the effects of bacteria to dissolution of rocks....
19 March 2020 316 4 View
I need to know which one, or if there is another, I could use to wash an intestine from a chicken, and then, to make serial dilutions. I was thinking to use saline sterile solution 0.9% to wash...
18 March 2020 3,196 4 View
I am searching for a solution containing both essential and non-essential amino acids. Mammalian cell culture seems to use these quite often, I would like to know if someone has experience using...
17 March 2020 7,836 3 View
I tried to do MIC tests on my two S. aureus strains, Methicillin-Susceptible S. aureus (MSSA) ATCC 25923 and Methicillin-Resistant S. aureus (MRSA) ATCC 43300. For the MIC test by broth...
03 March 2020 2,131 3 View
Which culture media is best for disk diffusion antibacterial susceptibility testing? Agar Bacteriological (Agar No. 1, Oxoid) or Müller-Hinton agar?
03 March 2020 4,402 3 View
Initially, when APTES was treated with glutaraldehyde, red colouration was observed. Then similar test was carried out with APTES and glutaraldehyde second time but the colouration was yellow. Why...
23 February 2020 9,154 5 View
Im working on production cellulose from isolate of Actenobacter which is im sure it produce cellulose, also im using a optimum conditions temp, media and static incubation , but there is growth...
22 February 2020 8,244 8 View
I am trying to grow Pseudomonas fluorescens ATCC13525 on different carbon source except glucose in M9 medium but i cannot see them growing on acetate. I am not able to find more information about...
21 February 2020 2,600 6 View
Any one can suggest some related ideas on the basis of gut microbiome, as i am having interest in it.. i am looking for one of my objective in microbiome study for my PhD thesis..
20 February 2020 2,226 4 View
Is it possible to determine the antibacterial minimum inhibitory concentration (MIC) using a liquid inhibition assay (MIC) with Lysogeny broth medium instead of Mueller-Hinton broth? If so, is...
10 February 2020 6,466 5 View
I am developing a proposal that will be testing on cytotoxicity of TfCRM107(derivative of Diphtheria Toxin) in vitro. Where or how can I acquire the immunotoxin?
06 February 2020 1,557 0 View
For patients with GI issues with the large intestine (IBS, spastic colon, ulcerative colitis), I have seen a pattern that foods with carageenan and xanathan gum, both polysaccharides used as...
05 February 2020 9,514 3 View
My overnight cultures of P. aeruginosa PAO1 can range from a creamy yellow colour to a bright green - what could be causing the different pigment production? I always prepare the cultures the...
03 February 2020 7,373 3 View
I am trying to isolate electrochemically active bacteria from activated sewage sludge, what composition should be good to use in my culture medium?
03 February 2020 7,443 2 View
I want to publish my research in a microbiology journal which publishes a research article without any charge. Could anyone suggest such journals?
02 February 2020 3,306 3 View
02 February 2020 9,817 3 View
02 February 2020 4,942 3 View
I am having difficulty finding the protein from yeast? Just want to extract protein and detect it by biuret reagent methods. I stored my yeast samples at -20C. So my question is how can I rupture...
02 February 2020 2,841 8 View
This is my first time working with thioglycollate broth and based on everything I've found I am not a 100% sure what this type of growth represents. I can see a growth on the top, then a line of...
02 February 2020 2,633 7 View
I would like to make a liquid medium anaerobic, how can I do this without an aneroid chamber or purging with anoxic gas? Also, I am attempting to use both facultative and obligate anaerobes,...
02 February 2020 6,722 3 View
02 February 2020 7,221 5 View
Hey everybody, I am looking for microbiology journals who publish opinion or perspective papers of not-so-high impact. I would like to publish an opinion about Raoultella spp. Any...
02 February 2020 5,374 3 View
I want to isolate fungi from sea water. Which medium do you recommend to do so? I was thinking about PDA modified with sea water but I am not sure if I should use either 100% sea water or a...
02 February 2020 1,650 3 View