Microbiology is a broad term which includes virology, mycology, parasitology, bacteriology, immunology and other branches. | Contact experts in Microbiology to get answers
3,365 views 3,642 posts
Questions related to Microbiology
I have RNAseq data made with selection by PolyA and other RNA-seq Total data. I want to join this data to increase the sampling within some subtypes I have few samples. How can I normalize these...
10 April 2023 4,983 2 View
Hello, Hope you are well. I am working on production of bacterial cellulose. I am finding difficulty in culturing of Acetobacters specific for production of bacterial cellulose. Anyone can guide...
08 April 2023 2,398 2 View
How does agriculture play an important role in economic development of India and Wdoes agricultural activities contribute to?
07 April 2023 6,915 2 View
I want to do liminology of my fish pond. My major concern is with different types of algae present in the pond. ....kindly share your experience regarding sampling of algae and cell count per...
05 April 2023 10,000 1 View
biofilm and quorum sensing genes of E.coli, not used drug but chemical material (Thiophene)
04 April 2023 931 11 View
What methods reduce microbiological pollution and effective method used in removing water borne micro organisms from bottled water?
03 April 2023 6,694 6 View
Hello multivariate gurus, I have a question regarding RDA multivariate analysis. Typically this is used to explain variation in a species/abundance dataset using envinonmental variables as a...
31 March 2023 5,636 5 View
I had test the killer effect of the yeast and I found some colonies seem to have an misty inhibition zone rather than a clear inhibition zone on plate as you can see in the picture. Is there any...
30 March 2023 9,008 14 View
I recently met an issue when GFP wasn't expressing in OE plants.. I want to know is there any method to analyze before agrobacterium transformation in Arabidopsis, whether my construct is working...
27 March 2023 4,053 3 View
Dear all, I am currently trying to determine the number of archaea present in an ecosystem with the 16S rRNA gene. I am normalizing the mcrA gene with the total archaea 16S rRNA gene. The mcrA...
17 March 2023 1,659 4 View
How does agriculture play an important role in economic development of India and reduce the impact of agriculture on the environment?
15 March 2023 6,587 7 View
I am currently working on the synthesis of chitosan-copper nanocomposite for environmental remediation. I attempted to disperse the nanocomposite in distilled water and some buffers; while the...
13 March 2023 8,136 3 View
I am using Roche Lightcycler 96 machine to perform protein thermal shift assays. I added 15 uL of 50 uM protein and 5 uL of SYPRO ORANGE fluorescent dye in DMSO. The temperature ranged from 30 °C...
13 March 2023 1,293 1 View
I will work MDA-MB-231 cell line in my thesis project and I use these mediums for the cell line. I hope that you answer my question.
04 March 2023 438 1 View
Hello, I made a cell lysate with a gram-positive bacterium using lysozyme and sonication. I need to inactivate the lysozyme because we are using the lysate in cell-stimulation assays and do not...
24 February 2023 5,022 1 View
My aim is to look at cytokine response (IL-1b, TNF-a, IL-6) in THP-1 cell supernatant after infection with GBS. Briefly, I inactivated GBS using formalin and incubated differentiated THP-1 cells...
23 February 2023 1,084 0 View
I got an invite to join SciPinion and I can't tell if it is a predatory journal or not. Anybody have experience with them? The email opened with this: "Your peers have identified you as an expert...
15 February 2023 5,274 6 View
Prodigiosin extracted from Serratia, Boiling point- 554.3 Celcius, MW- 323.4 Da
11 February 2023 1,561 2 View
We are working on Biofilm and Quorum sensing genes of E.coli, we suggest some of reasons maybe:- Contamination, Annealing Temperature, Melting Temperature, DNA Extraction mistakes in the...
10 February 2023 7,090 10 View
other than DNA gel extraction
10 February 2023 8,375 0 View
This is my research topic and I appreciate any help in exploring this issue further.
08 February 2023 1,468 2 View
What is the best method for DNA isolation from fish samples? Any experience and/or tips how to improve yield of genomic DNA from fish samples? What tissue is the best source? How much DNA is...
01 February 2023 5,807 1 View
I have a protein sequence with two cysteine residues and I would like to predict if those cysteins will form disulfide bonds. I am looking for user-friendly tools to do this, either online tools...
19 January 2023 1,587 4 View
I have protein spectra, and I want to calculate the protein's secondary structure..... is it possible to calculate % of alpha, beta, and random content without instrument link software. like any...
19 January 2023 3,961 3 View