Microbiology is a broad term which includes virology, mycology, parasitology, bacteriology, immunology and other branches. | Contact experts in Microbiology to get answers
1,811 views 3,290 posts
Questions related to Microbiology
Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...
25 February 2021 3,531 6 View
I am using streptomycin to kill the extracellular bacilli while infecting THP1 derived macrophages with M.tb. Can streptomycin cross the macrophage membrane ?. I read some articles. It says that...
16 February 2021 1,987 3 View
I am aware of a study that has compared the use of citrated and defibrinated sheep's blood in microbiological media (Hair Sheep Blood, Citrated or Defibrinated, Fulfills All Req... ), but can...
11 February 2021 6,210 1 View
How can I know the stationary phase of growth of several bacteria? We are aiming to collect exoproteome of several bacteria. We know that the ideal time to collect exoproteome is during the...
01 February 2021 4,900 10 View
I have tried to use TTC agar overlay method for a few times to identify respiratory-deficient yeast which were affected by EthBr. The problem is that cells which should have stayed white also have...
23 January 2021 764 10 View
Need to check quorum sensing activity of Vibrio campbellii.
21 January 2021 791 5 View
I have an unstained section in the PEN membrane slide already and need to stain it with Cresyl Violet Stain to isolate RNA/DNA, do I directly put the solution on the section or do I dehydrate...
07 January 2021 716 0 View
I received several strains of bacteria from another lab far away. However, one of the samples appears to have high fungal contamination. When I grow it in broth, I see lots of fungal...
04 January 2021 2,963 16 View
According to the popular model proposed, Cry1A toxin binds with Aminopeptidase N to form pores in the membrane. How the mechanism for binding toxins to receptors and how these receptors change the...
04 January 2021 7,673 2 View
Hi, I'm expressing and purifying a membrane protein which at the C-terminus has a short linker followed by a TEV-mEmerald-TwinStrep tag. I can solubilise and purify the protein with...
02 January 2021 2,418 7 View
the study of microorganisms is called microbiology Microbiology is the study of microscopic organisms, such as bacteria, viruses, archaea, fungi and protozoa. This discipline includes fundamental...
14 December 2020 8,526 6 View
According to the Baier minima, surfaces having critical surface tensions between 20-30 mN/m are considered as foul release surfaces. PDMS or silicone material falls under this range according to...
11 December 2020 3,613 2 View
I am working on virulence factors and proteins associated with H. pylori infection. For this, I have collected gastric biopsies and treated them with T-PER Tissue Protein Extraction Reagent (...
07 December 2020 3,674 2 View
We want to screen halotolerant protease producing bacteria. Since Skim milk (HiMedia) is available in our lab, therefore, we want to screen extracellular protease by this compound/ supplement. We...
05 December 2020 7,831 6 View
Microbiologist’s opinion needed Hello everyone, Short version: We want to study microbiologically influenced corrosion in simulated seawater. How do we cultivate marine bacteria in the...
03 December 2020 6,932 3 View
How long is it possible to store the bacterial culture at 4C?
03 December 2020 2,922 11 View
Do you have to normalize microbiome abundance data prior to running an NMDS ordination? I am getting very confused. In the data processing stage, I subsampled the OTU table to a certain read count...
03 December 2020 165 0 View
I know that fixation with cold Methanol permeabilizes the cell membrane and that there can be a leakage of metabolites. I wonder if there is a chance that also proteins leak out of the cell? We...
26 November 2020 2,170 0 View
I've been trying to recover some bacterial cells by streaking them on agar plates, without success. The stock has been kept in good conditions. But, there is a white pellet at the end of the...
25 November 2020 5,739 8 View
For Fungal Spore suspension, can I use Tween 20 instead of Tween 80 as both are detergents?
20 November 2020 9,475 2 View
Hello. I would like to find a way to calculate the Units (not in log cfu/g or log cfu/ml) of my lyophilized kefir culture. Thank you
09 November 2020 8,570 3 View
I have only 25 S.aureus isolates from bovine mastitis (only 3 showing some antibiotic resistant properties). Considering the number to be not enough to publish a paper on genotypic and phenotypic...
08 November 2020 6,925 4 View
I found this organism in my Petri dish following soil serial dilution. It is embedded in the media and is showing a branched- like pattern. I am relatively new to microbiology and I can not figure...
05 November 2020 1,919 11 View
I have prepared PBS buffer and components for M9 medium and I store it in freeze, I do not use it if it is more than 2 weeks old. From time to time I check the sterility of these solutions and...
05 November 2020 9,659 6 View