Stable extracellular enzyme activities are associated with soil colloids and persist even in harsh environments that would limit intracellular microbiological activity. Thus, only strictly intracellular enzyme activities can truly reflect microbial activity because the contribution of free extracellular enzyme released by active microbial cells is negligible; indeed, these enzymes are shortlived because they are degraded by proteases unless they are adsorbed by clays.

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