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Questions related to Microbiology
I saw it in the laboratory and it was like Serratia marcescens, but I was sure it is Moraxella.
27 November 2023 5,576 0 View
Hi everyone! I was hoping anyone can point me to suppliers of hydrothermal synthesis autoclave reactors. Perhaps some cheap but quality ones because I have to purchase them by myself. Thank you...
23 November 2023 6,067 1 View
Where can I do a gut microbiome profiling in the UK? It is for my PhD study. I need to do a shallow shotgun sequencing using faecal samples. Can anyone one recommend a good company who does this...
22 November 2023 809 1 View
In some iterations, when a new column is added to the master, it does not enter the base and a stop condition occurs, or a new column with a better objective function is found. is it correct? When...
21 November 2023 8,641 0 View
This should be for a research on access.
21 November 2023 3,922 0 View
I'm facing difficulties while preparing double agar using the bacteria I initially used to isolate my phage. I wanted to see how different concentrations of these bacteria (measured as OD 0.1,...
17 November 2023 5,742 2 View
I prepare a soluble microneedles loaded with nanoparticles, in the release experiment i centrifuge the withdrawn sample at predetermined time point, in order to take the free drug from...
06 November 2023 7,240 0 View
Hello Everyone, I have one question about the concentration of LPC for ADA assay. When we validation the confirmatory assay, we need the LPC concentration? Thank you very much in advance! QL
05 November 2023 8,655 1 View
Dear ResearchGate community, I have a question regarding the possibility of a batch effect in my single-end bulk RNASeq data set: Some of my samples (10 out of 30) were sequenced 2x due to...
02 November 2023 6,975 1 View
Neurons were treated with four different types of drugs, and then a full transcriptome was produced. I am interested in looking at the effects of these drugs on two specific pathways, each with...
01 November 2023 2,610 5 View
The question was answered independently. Thank you for your replies.
27 October 2023 1,901 3 View
Do some journals accept the manuscripts of scholars, who have a lot of publications, more quickly than beginners, who have no or less number of publications, irrespective of the quality of the...
22 October 2023 8,702 1 View
Dear All, I want to make a side-chain stapling of lysine (or its non-standard amino acid derivatives). How to make a topology file for this kind of side-chain stapling (I believe, it is not...
20 October 2023 8,241 2 View
Identification, characterization and selection of autochthonous lactic acid bacteria as probiotic for feedlot cattle
20 October 2023 4,682 0 View
Our Eppendorf 5430R centrifuge is making a high pitched noise. Rotor has been cleaned. What is causing this?
19 October 2023 990 3 View
Hi everyone, I am working on goat semen using quercetin as an antioxidant but not getting proper post-thaw sperm motility (40 to 60%). Sperm wave motion above 4 and sperm motility more than 80%....
03 October 2023 5,137 11 View
We are using Bio-Rad RAPID'Salmonella agar (prepared from powder media) for Salmonella spp confirmation. We recently switched over from pre-poured plates directly from the manufacturer to making...
30 August 2023 4,786 7 View
Hello everyone, I have prepared SnO2 nano powders using chemical co-precipitation method. Eventually, I want a colloidal solution of SnO2. Please guide me how I can convert the nanopowder to...
24 August 2023 7,571 6 View
I am expressing the protein in the E. coli host cell, followed by the purification using dialysis buffer (20 mM phosphate buffer, 100 mM NaCl, 2 mM EDTA, 0.01% sodium azide, pH 7) for 12 h at room...
23 August 2023 5,022 2 View
I know many websites have simple tools like transcription and translation available, but are there any analysis tools that researchers need that either do not exist or are not publicly available?...
29 July 2023 8,644 3 View
Hi, I have been culturing bovine digest satellite cells recently. In my very latest study, i seeded it at 2.65 million cell in T75 with dmem/f12+20% fbs and 1% antian and 10ng/mL fgf and tried...
25 July 2023 9,474 0 View
Hello 1- in Parkinson research , we have 40 male, C57/BL mice each 26 mg they will received 10 doses of 1-methyl-4- phenyl-1,2,3,6-tetrahydropyridine,MPTP, 25 mg/kg, s.c. (MPTP),how much mptp...
17 July 2023 1,617 0 View
How do scientists use metagenomics and high-throughput sequencing technologies to study microbial communities and uncover novel microbial species and functions?
15 July 2023 8,472 1 View
I am running ELISAs. I run blank samples, without standard, and I subtract the mean OD value from the blank wells from the ODs of the other wells (standards and samples). Nevertheless, I have...
13 July 2023 9,179 3 View