I want to know how to make probiotic in different concentration. I am planning to probiotic experiment with different concentration. I also want to know how to make the freeze dry probiotic powder with a definite concentration.
Using McFarland standard we can workout for the turbidity matching for a particular number of cells per ml by performing TVC and use that concentration for the desired experimentation. The same can be use for freeze dry powder preparation by the method of choice.