Microbiology is a broad term which includes virology, mycology, parasitology, bacteriology, immunology and other branches. | Contact experts in Microbiology to get answers
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Questions related to Microbiology
biofilm and quorum sensing genes of E.coli, not used drug but chemical material (Thiophene)
04 April 2023 825 11 View
What methods reduce microbiological pollution and effective method used in removing water borne micro organisms from bottled water?
03 April 2023 6,633 6 View
Hello multivariate gurus, I have a question regarding RDA multivariate analysis. Typically this is used to explain variation in a species/abundance dataset using envinonmental variables as a...
31 March 2023 5,547 5 View
I had test the killer effect of the yeast and I found some colonies seem to have an misty inhibition zone rather than a clear inhibition zone on plate as you can see in the picture. Is there any...
30 March 2023 8,920 14 View
I recently met an issue when GFP wasn't expressing in OE plants.. I want to know is there any method to analyze before agrobacterium transformation in Arabidopsis, whether my construct is working...
27 March 2023 3,951 3 View
Dear all, I am currently trying to determine the number of archaea present in an ecosystem with the 16S rRNA gene. I am normalizing the mcrA gene with the total archaea 16S rRNA gene. The mcrA...
17 March 2023 1,568 4 View
How does agriculture play an important role in economic development of India and reduce the impact of agriculture on the environment?
15 March 2023 6,508 7 View
I am currently working on the synthesis of chitosan-copper nanocomposite for environmental remediation. I attempted to disperse the nanocomposite in distilled water and some buffers; while the...
13 March 2023 8,064 3 View
I am using Roche Lightcycler 96 machine to perform protein thermal shift assays. I added 15 uL of 50 uM protein and 5 uL of SYPRO ORANGE fluorescent dye in DMSO. The temperature ranged from 30 °C...
13 March 2023 1,201 1 View
I will work MDA-MB-231 cell line in my thesis project and I use these mediums for the cell line. I hope that you answer my question.
04 March 2023 357 1 View
Hello, I made a cell lysate with a gram-positive bacterium using lysozyme and sonication. I need to inactivate the lysozyme because we are using the lysate in cell-stimulation assays and do not...
24 February 2023 4,935 1 View
I got an invite to join SciPinion and I can't tell if it is a predatory journal or not. Anybody have experience with them? The email opened with this: "Your peers have identified you as an expert...
15 February 2023 5,180 6 View
Prodigiosin extracted from Serratia, Boiling point- 554.3 Celcius, MW- 323.4 Da
11 February 2023 1,471 2 View
We are working on Biofilm and Quorum sensing genes of E.coli, we suggest some of reasons maybe:- Contamination, Annealing Temperature, Melting Temperature, DNA Extraction mistakes in the...
10 February 2023 6,956 10 View
other than DNA gel extraction
10 February 2023 8,298 0 View
What is the best method for DNA isolation from fish samples? Any experience and/or tips how to improve yield of genomic DNA from fish samples? What tissue is the best source? How much DNA is...
01 February 2023 5,716 1 View
I have a protein sequence with two cysteine residues and I would like to predict if those cysteins will form disulfide bonds. I am looking for user-friendly tools to do this, either online tools...
19 January 2023 1,495 4 View
My blood agar plate turns to chocolate agar plate after being incubated at 37 degree Celsius for 24 hours. I cooled down the NA agar to 50 degree before adding the defibrinated sheep blood. What...
18 January 2023 8,339 0 View
We are utilizing an aluminum 96well plate system with glass vials due to organic we are utilizing. We need to heat the system to 100C and not let any organics escape. We are having issues with...
10 January 2023 2,933 1 View
Why primer binding template instead of the two strands bind each other? The Tm of one strand is higher than primers. Complex structure of the template? The amount of primer is much higher than...
29 November 2022 5,747 1 View
Hi everyone, I am trying to grow marine cyanobacteria from my samples using BG11 and L1 as nutrient and agar as gelling agent. I found agarolytic bacteria thrives in my plates, liquifying my...
28 November 2022 5,332 3 View
Hi, i have now a fully working panel with lots of markers and will proceed to stain and compare samples, pmbc healthy vs non using flow cytometry. Which one of these programs to choose for...
23 November 2022 5,877 4 View
I tried to tosylation glycerol carbonate with sodium hydride base in THF solvent. but C=O bond in glycerol carbonate was broken. can you help me what is the reason of that? is it becauce of the...
15 November 2022 1,749 4 View
although we have already used f8 and r1510 primers but the results are not enough good
14 November 2022 6,856 0 View