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Questions related to Microbiology
I am trying to run an RDA or CCA (Redundancy Analysis, And Canonical Correlation) to illustrate the relationship between measured gene abundance and a set of env. variables. The environmental...
01 November 2022 8,239 4 View
The objective is to sequence two alleles, and PCR products are amplified in the same PCR, but better results are generally obtained for the shortest PCR product. How can I optimize reaction...
19 October 2022 6,012 4 View
I am preparing gastrointestinal fluids to test yeast survivability in-vitro. The protocol requires me to prepare two solutions, gastric juice and intestinal fluid, requiring me to add pepsin and...
18 October 2022 8,679 5 View
Hello, I've been tasked with validating a 2kb region of DNA. A colleague designed primers with the F melting at 53.8 and the reverse at 59.7. I believe they used 54C which had multiple bands show...
17 October 2022 5,366 6 View
How to set the protocol and how to set all parameters for the experiment?
13 October 2022 8,307 3 View
We intend to perform a Galleria mellonella infection assay, infecting them with clinical strains of Klebsiella pneumoniae, then treating them with two antimicrobials sequentially applied. Have you...
28 September 2022 8,548 1 View
Since, HepG2 is a non-tumorigenic cell line, if i inject it into a nude mice will the cancer be induced by the presense of it?
13 July 2022 7,431 1 View
Dear all, I have to draw the complete pedigree of a high number (>40) of dogs in a study regarding genetic diseases, so I started drawing the pedigree using the R package kinship2. The...
30 June 2022 221 3 View
We are looking for explanations of the differences between buffaloes and cattle, we have obserced low expression of FSHr transcripts between cattle and buffaloes, but we don´t have data to compare
27 June 2022 8,978 5 View
hey, i isolated from kefir drink .. after purification i tried to recognize the colonies under microscope but i found different colony ! any idea about it ?
21 June 2022 7,175 1 View
I am trying to bind lipid extractions as well as purified single lipids from mycobacteria to Nunc PolySorp 96 well plates to perform ELISA's. Lipids are insoluble in water, so when I try and...
25 April 2022 7,501 4 View
To give context, I'm trying to keep the cells in an anaerobic environment by sealing the plate with mineral oil and transparent 96 well plate seals. But, the bacteria don't grow once they're out...
24 April 2022 6,676 0 View
I have transformed at 0.3 OD but callus is not survived in selection media.
04 April 2022 9,301 6 View
Which Level (L1 or L2) of LANDSAT 8 OLI images is best for estimating soil salinity?
08 March 2022 5,995 4 View
While determining the oxidation% of my PDA solution in my hydrogel project, I am suddenly curious why we do this, this way.
23 February 2022 3,828 3 View
Biofilm bacteria show increased resistance to toxic heavy metals. The remarkable tolerance is often attributed to the EPS matrix. Several studies have confirmed the involvement of protein like...
18 February 2022 9,553 0 View
Hey I was wondering if anyone has any ideas as to why I ended up with very small quantities of finished library after starting with 1 ug total RNA per sample and after following the low sample...
26 January 2022 1,268 0 View
I need to make a larger quantity of formalin than usually used in my lab. The protocol I have for this requires glassware. I want to know how to clean/decontaminate this properly so it can safely...
04 January 2022 7,933 1 View
I would like to analyse the visual cells (cone and rod cells) of the retina of tuna species. The eyes are fixed in 10% neutral buffered formalin. I would appreciate to know if there is a...
23 November 2021 7,265 5 View
Could anyone inform me on the real danger of ethidium bromide? I know that it is mutagenic but is there any proof that it might also be carcinogenic? If yes, which kind of contact with ethidium...
15 November 2021 9,391 4 View
Hi there, I recently started working with the VCaP cell line. I used ATCC recommended media to propagate those cells. I am having a hard time propagating them. Can someone recommend the media...
28 September 2021 7,327 2 View
Please recommend online tools or software that can be used for designing synthetic alpha-helix peptides. Thank you.
06 September 2021 4,896 2 View
I measured the OD of my samples using spectrophotometer (750 nm), and microplate reader, and there was a difference between them.. so when do I consider the difference a minor and neglectable.....
25 August 2021 1,485 2 View
With what software can I carry it out? Does anyone have a paper or guide that can be indicated to guide me? I greatly appreciate your responses.
09 August 2021 9,631 3 View