Mice

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Questions related to Mice

How long can unstained fixed (1.5%PFA for 30 mins) Bone Marrow cells be stored in 4°C. Can it be stored in -80°C for better preservation?

I have fixed bone marrow cells of mice in 1.5% PFA for 30mins and after washing stored them in 4°C resuspended in FACS fluid. It's already been 2 weeks now and flow cytometric analysis will not...

03 March 2022 6,300 2 View

What are the proper query selection criteria for performing homology search of peptides (45 aa) using Blastn?

Hi, We are dealing with bioactive peptides of around 45 aa residues from crustaceans. I am planning to search for homolog peptides in other crustacean species using blastn. My doubt is whether...

03 March 2022 6,522 1 View

How to increase cell concentration after FACS with a small cell number?

Hello! I have been trying to increase cell density after FACS. I was sorting retina ganglion cells from adult zebrafish retina. I can get 15k cells in 40µl AMES. I put this directly to centrifuge....

03 March 2022 5,653 3 View

What percentage of Baseline you concider as LTP in mice?

I'm working on taking LFP signal from the hippocampus of mice brains for my project, So I wonder what percentage of the baseline signal you would consider as LTP? and what is the best period of...

03 March 2022 2,351 3 View

How to precipitate bacterial outer membrane proteins?

I wish to purify the outer membrane proteins of E. coli. The methods available involve the ultracentrifugation as mentioned by Choi-Kim et al. (1991). I wish not to use ultracentrifugation. Can...

02 March 2022 8,991 5 View

How to induce NETs with activated platelet?

I'm doing experiments on neutrophils producing NETs. In the preliminary experiment, I successfully induced NETs from rat neutrophils using PMA. When I used ADP preactivated platelets to stimulate...

28 February 2022 4,551 7 View

Hi! Do anyone knows or have a social skill scales that I can access for free?

I'm currently conducting a study about social skills and academic performance of students.

28 February 2022 8,642 3 View

How to present an Interpretive Phenomenological Analysis (IPA) study?

While using Interpretive Phenomenological Analysis (IPA) in a Qualitative study, how can we represent data from several stakeholders other than the target group to explain different perspectives...

28 February 2022 8,606 5 View

I'd like to ask about reagents used for SA-b-gal staining?

Potasium ferrocyanide and postasium ferrocyanide are used in SA-b-gal staining experiments, and I wonder what the two reagents play. I think it's going to play a very important role.

26 February 2022 7,452 1 View

4-OH-TAM in ALI?

Hello- I am trying to use 4-OH-TAM in mouse ALI culture and it doesn't seem to be working. Tips on what concentration to use and how to prepare would be much appreciated

24 February 2022 9,445 3 View

Cutting mouse bones on microtome for P-IHC?

I am having troubles cutting paraffine embedded mouse bones on a microtome. Fixation and embedding can't completely remove calcium, and I end up with thorn slices all the time. Even additional...

24 February 2022 8,842 3 View

How can I remove EDTA from plasma sample?

I want to remove EDTA from the mice plasma samples. I know that there are some dialysis cassettes available in the thermo to do it but they have not mentioned about the buffer required. Has...

23 February 2022 1,662 1 View

Hi, Can anyone share an established protocol for brain slice cultures?

We are trying to do some slice cultures and we need an established protocol for cultures. Also how transduce the slices with AAVs ?

23 February 2022 1,137 1 View

Does anyone know a method to chemically deplete Leydig cells in adult male mice?

Dear all, does anyone know a method to chemically deplete Leydig cells in adult male mice? Thank you very much. David

22 February 2022 4,096 1 View

Flow cytometry gating strategy for mouse blood monocytes?

I have stained peripheral blood from mice with CD45, CD11b, Siglec F, Ly6G, Ly6C, CX3CR1 and CD64 antibodies However, I am unsure how to gate for monocytes only using these markers. It seems...

22 February 2022 5,231 3 View

How to analyze RNA seq RPKM values?

I have received RNA seq data from three KO mice and wild type mice. The mice are littermates from commom father and two mother and age of 2-month old. I got the RPKM values and performed GSEA...

22 February 2022 7,430 1 View

How can I prevent my aortic frozen sections embedded in OCT compound to fall off the slides while hematoxylin eosin staining protocol?

I have tried fixing in PFA 4% for an hour followed by 2 PBS washes, it has worked but not all the times and I end up losing valuable samples. I have seen comments that indicate to do what I´ve...

22 February 2022 7,865 6 View

How to validate Novel object recognition objects?

I am planning on completing novel object recognition testing in C57 mice but I need to validate the objects in a separate cohort to ensure there is not innate preference to any object. Does anyone...

21 February 2022 3,788 3 View

Why 5-ASA did not show anti-inflammatory activity in DSS-induced colitis mice model?

I tested 1 compound and 5-ASA (as positive control) in the DSS-induced colitis mice model. Neither my compound nor 5-ASA showed anti-inflammatory activity. In fact, 5-ASA was a bit...

19 February 2022 6,321 3 View

What dilutions of serum sample should I use in ELISA?

I would like to do an ELISA to measure the levels of a biomarker in mouse serum. The concentration of the standards ranges from 2000pgs/ml to 31.25pgs/ml. I have the kit from R&D systems but...

19 February 2022 9,998 2 View

What's the best way to transfer solution into a dialysis tube?

Greetings everyone, I would like to ask those who have worked with dialysis tubes similar to the one pictured for advice on the best way to keep the dialysis tube upright while transferring a...

18 February 2022 6,548 2 View

How do I use stereotactic brain injection to administer multiple viruses at once in the same mouse?

I want to inject multiple viruses in different brain regions of the same mouse. But the mouse's brain cannot withstand the damage of multiple needle injections. Thanks, July

16 February 2022 9,646 3 View

What is the most significant expression of superior semicircular canal dehiscence that you have seen?

In those cases, what treatment decisions did they make? What variables did they consider?

16 February 2022 628 4 View

How do I clean endotoxin from plastics?

Hi, I'm looking to do an endotoxin-free plasmid DNA purification using QIAGEN EndoFree plasmid maxi kit. I need to use endotoxin free pipette tips and tubes for this. For the centrifugation steps...

16 February 2022 9,748 3 View

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