09 August 2024 0 5K Report

Hello;

I have been able to isolate CD34 cells from cord blood, sadly this is our 31st attempt. We have tried to immortalize CD34 cells through Retronectin infusion, removing it and then sandwiching, CD34 cells with HPV16 E6 and E7. Sadly after Day 3 of Expansion the cells start to die, and a lot of debris is seen. Our immortalization aspect is not working at all. Any advices?

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