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Questions related to Mice
In patients treated with incretin-based agonists such as Semaglutide or Tirzepatide, weight loss often reaches a plateau after an initial period of reduction. What are the physiological,...
3 weeks ago 3,701 2 View
Banking concentration negatively affects financial inclusion in Nigeria by increasing the cost of financial services and limiting access for the unbanked population, as large, concentrated banks...
3 weeks ago 3,085 3 View
The following task to be evaluated are; Letter Writing Essay Writing Summary Writing
4 weeks ago 4,330 5 View
"I'm optimizing the control group OD for an MTT assay on Leishmania major promastigotes in 96-well plates to establish a suitable seeding density for drug screening. I seeded different parasite...
4 weeks ago 5,132 1 View
Gel type: Novex 4–12% TG WedgeWell precast gel Running conditions: 70 V for 15 minutes (stacking), then 105 V until the dye front reached the bottom Protein load: 5 µg per lane I’m new to Western...
4 weeks ago 1,608 1 View
Dear Colleagues, I’m conducting PhD research in English Language Teaching and have developed the Peer Review Lab — a self-paced, 10-module online training to build peer review skills in foreign...
5 weeks ago 2,742 2 View
I want to write a review paper on specific topic in pharmacy, how I can know the novelty of topic & the number of review papers. How I can choose a novel title in pharmacy field?
last month 3,812 1 View
I isolated RNA from pooled samples of mice retina (frozen samples). The RNA quality was fine. But, I am not able to detect the expression of a particular gene while the others were able to detect...
last month 6,558 1 View
How can this problem be solved?
last month 8,599 3 View
I am a researcher I have recently completed my phd in thermochemical conversion of ionic liquid pretreated spent coffee ground . In order to enhance my research experience; I need collaboration...
last month 1,991 5 View
1. Are there specific vectors available that help prevent false-positive results during cloning?
last month 2,304 3 View
I would like to biotinylate a recombinant protein via its primary amines. Can plain D-biotin be used directly, or does it need to be converted into a reactive form such as NHS-biotin? If...
last month 306 3 View
Hello everyone, I'm working on a viral genomics project and have encountered an interesting assembly result that I'd appreciate some professional advice on. I have sequenced the genome of an...
last month 3,720 0 View
How to start a model on homogeneous isotropic turbulence flow model in cp3d.How we get data first by using cp3d for this model.
last month 4,574 1 View
I tried to isolate endothelial cells from murine lungs with sorting method and I use antibody coated Dynabeads for the magnetic separation. As I plate my cells for culture in 24 well plates, the...
11 August 2025 939 1 View
I have a nutrition experiment in which I will use laboratory rats. The number of rats is large, 80 rat. I want to estimate insulin at the beginning and end of the experiment, but the cost is high....
04 August 2025 3,386 3 View
I am trying to figure out how to use MitoSox green to measure the superoxide levels in human cerebral microvascular endothelial cells (HCMEC). For most ROS kits you can use a microplate reader to...
31 July 2025 4,388 4 View
from left to right are the constant current charge-discharge curves of materials a) CoFe2O4, b) CoFe2O4@AC 10%, and c) CoFe2O4@Cell 10% synthesized by the co-precipitation method. Please help me...
28 July 2025 8,008 4 View
Application of Bayesian statistics in healthcare data analysis.
18 July 2025 8,915 1 View
I am trying to determine the Michaelis-Menten Constant. However, as the concentration of my peptide substrate increases, the RFU signal started to mess up and does not follow the typical kinetic...
30 June 2025 4,237 7 View
Could you please advise on the optimal method for dissolving recombinant proteins and any important precautions to consider during the process? I have purchased a lyophilized recombinant protein...
26 June 2025 7,095 5 View
I have cloned pET28a vector with BF AchE gene, the recombinant pET 28a vector is transformed in BL21 cells and for purifying His-tagged BF AchE enzyme, Please suggest the lysis buffer that I can...
21 June 2025 3,131 2 View
I am currently trying to do some analysis for the Research in Research project using acoustic emission. The issue I am having is that the WFS data, using several code DLL libraries to convert to...
19 June 2025 9,326 0 View
Any relevant paperwork or publications are highly expected! Thanks in Advance
17 June 2025 5,562 4 View