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Questions related to Mice
In patients treated with incretin-based agonists such as Semaglutide or Tirzepatide, weight loss often reaches a plateau after an initial period of reduction. What are the physiological,...
26 September 2025 3,745 2 View
Banking concentration negatively affects financial inclusion in Nigeria by increasing the cost of financial services and limiting access for the unbanked population, as large, concentrated banks...
24 September 2025 3,113 3 View
The following task to be evaluated are; Letter Writing Essay Writing Summary Writing
20 September 2025 4,349 5 View
"I'm optimizing the control group OD for an MTT assay on Leishmania major promastigotes in 96-well plates to establish a suitable seeding density for drug screening. I seeded different parasite...
19 September 2025 5,148 1 View
Gel type: Novex 4–12% TG WedgeWell precast gel Running conditions: 70 V for 15 minutes (stacking), then 105 V until the dye front reached the bottom Protein load: 5 µg per lane I’m new to Western...
15 September 2025 1,628 1 View
Dear Colleagues, I’m conducting PhD research in English Language Teaching and have developed the Peer Review Lab — a self-paced, 10-module online training to build peer review skills in foreign...
13 September 2025 2,783 2 View
I want to write a review paper on specific topic in pharmacy, how I can know the novelty of topic & the number of review papers. How I can choose a novel title in pharmacy field?
11 September 2025 3,830 1 View
I isolated RNA from pooled samples of mice retina (frozen samples). The RNA quality was fine. But, I am not able to detect the expression of a particular gene while the others were able to detect...
31 August 2025 6,689 1 View
How can this problem be solved?
20 August 2025 8,617 3 View
I am a researcher I have recently completed my phd in thermochemical conversion of ionic liquid pretreated spent coffee ground . In order to enhance my research experience; I need collaboration...
20 August 2025 2,005 5 View
1. Are there specific vectors available that help prevent false-positive results during cloning?
20 August 2025 2,324 3 View
I would like to biotinylate a recombinant protein via its primary amines. Can plain D-biotin be used directly, or does it need to be converted into a reactive form such as NHS-biotin? If...
19 August 2025 438 3 View
Hello everyone, I'm working on a viral genomics project and have encountered an interesting assembly result that I'd appreciate some professional advice on. I have sequenced the genome of an...
19 August 2025 3,743 0 View
How to start a model on homogeneous isotropic turbulence flow model in cp3d.How we get data first by using cp3d for this model.
18 August 2025 4,592 1 View
I tried to isolate endothelial cells from murine lungs with sorting method and I use antibody coated Dynabeads for the magnetic separation. As I plate my cells for culture in 24 well plates, the...
11 August 2025 952 1 View
I have a nutrition experiment in which I will use laboratory rats. The number of rats is large, 80 rat. I want to estimate insulin at the beginning and end of the experiment, but the cost is high....
04 August 2025 3,403 3 View
I am trying to figure out how to use MitoSox green to measure the superoxide levels in human cerebral microvascular endothelial cells (HCMEC). For most ROS kits you can use a microplate reader to...
31 July 2025 4,404 4 View
from left to right are the constant current charge-discharge curves of materials a) CoFe2O4, b) CoFe2O4@AC 10%, and c) CoFe2O4@Cell 10% synthesized by the co-precipitation method. Please help me...
28 July 2025 8,025 4 View
Application of Bayesian statistics in healthcare data analysis.
18 July 2025 8,969 1 View
I am trying to determine the Michaelis-Menten Constant. However, as the concentration of my peptide substrate increases, the RFU signal started to mess up and does not follow the typical kinetic...
30 June 2025 4,316 7 View
Could you please advise on the optimal method for dissolving recombinant proteins and any important precautions to consider during the process? I have purchased a lyophilized recombinant protein...
26 June 2025 7,152 5 View
I have cloned pET28a vector with BF AchE gene, the recombinant pET 28a vector is transformed in BL21 cells and for purifying His-tagged BF AchE enzyme, Please suggest the lysis buffer that I can...
21 June 2025 3,170 2 View
I am currently trying to do some analysis for the Research in Research project using acoustic emission. The issue I am having is that the WFS data, using several code DLL libraries to convert to...
19 June 2025 9,351 0 View
Any relevant paperwork or publications are highly expected! Thanks in Advance
17 June 2025 5,604 4 View