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Questions related to Mice
I have used different methods including graphpad prism, excel and various other online tools but i get different values each time. I want to know how IC50 can be determined from % inhibition and...
03 October 2022 1,091 4 View
When doing the indirect ELSIA experiment, I only added the antigen dilution to the wells without adding the corresponding antigen, but the results showed a high OD, the primary antibody was the...
27 September 2022 3,688 2 View
These images are from Bone marrow hMSCs and gingival fibroblasts. There are small vesicles around the cells and also inside the cells. in hMSCs you can see once the vesicle density is high cells...
26 September 2022 6,997 0 View
When I record the current of EAAT1 which is expressed in 293T cells,there is no current with the the presence of L- glutamic acid.
26 September 2022 7,119 0 View
The company that used to do it closed down so we need to find an alternative. Thanks in advance!
22 September 2022 7,618 0 View
is there any traditional method to check T cell levels or can we isolate T-cells to check Th17 cell levels using specific markers? I am a little stuck here. Just to clarify I don't want to use...
21 September 2022 1,255 1 View
Hi everyone! I'm a PhD student and I've been stuck for long time now with culturing regulatory T cells (Tregs) from the mouse spleen. I've made several attempts but currently I'm using this...
14 September 2022 827 0 View
I was wondering what an adequate stressor for late pre-weaning mice would be (postnatal day 17 to 18 - date of birth = postnatal day 0). I know for younger animals it is maternal separation and...
12 September 2022 4,816 0 View
Hello! Recently I sent some samples to a company for ChIP-seq against H3K79me2 and I want to compare the differentially methylated regions between four samples, two of them treated with the DOT1L...
11 September 2022 5,867 1 View
The test is developed by Power, M.J.; Green, A.M. and Published in: Journal of Intellectual Disability Research in the year 2010.
09 September 2022 3,193 2 View
homostasis if i deplete the macrophage,How NK cells change i wanna some related articles need your help!!! thank you ! love you
04 September 2022 8,907 1 View
A small group of People with Parkinson's disease, of which I am one, are doing some research to try to standardize a preparation method to make precise quantities of the isothiocyanate...
02 September 2022 7,599 0 View
Hi, I am running through chromatin immunoprecipitation. To reverse the cross-link after immunoprecipitation, the protocol calls for an overnight incubation at 65C. Due to some scheduling conflicts...
30 August 2022 1,127 1 View
Please, kindly help me with an optimized method of isolation of lactoferrin using CM-Sephadex-C50. Please, including the method of sephadex preparation from it solid form .
27 August 2022 1,354 1 View
The intention is to produce polyclonal IgY antibodies.
26 August 2022 738 2 View
Is there a review paper in arthritis just like "Hallmarks of cancer" in Cancer research?
25 August 2022 8,860 2 View
I ran an experiment to observe differences in risky decision-making between food and monetary rewards. For the group of participants assigned to the food rewards group, I asked them how hungry...
24 August 2022 496 1 View
What is the best method for providing non-degraded RNA after extraction. The direct-zol miniprep kit has been used as well as the TRIzol-chloroform method. Or what modifications can be made to...
23 August 2022 2,434 0 View
I have a project with human neural stem cells but the ones that I was planning to use is from ATCC and currently they are out of stock. Does someone has another company or commercial source to...
22 August 2022 4,889 2 View
I have a Caco-2 cell that I don't know about the passage to this cell. I'm observing that always in pass 8, after receiving these cells, it starts to present this phenotype in the culture (photo)....
22 August 2022 9,855 2 View
I start learning about synchronization between different states and criticality in Parkinson's disease. Would it be possible for someone to recommend a relevant and practical review paper that...
21 August 2022 4,048 4 View
I am looking out for lung cancer cell lines having high and low metastatic potential. While I found A549 to be highly metastatic, I could not find any one whose metastatic potential is low in...
21 August 2022 2,732 0 View
Hello everyone, I've been trying to find an article of a standarized protocol for subculturing THP-1 macrophages, however I haven't found anything about that, does anyone know what is best for...
19 August 2022 9,190 2 View
I am trying to test whether certain compounds we have formulated exhibit antimicrobial activity and I am using Whatman 6mm assay discs. I want to sterilize them in the autoclave but after wrapping...
18 August 2022 7,743 5 View