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Questions related to Mice
I am working on invitro fertilisation in a selected Indian breeds. Performing IVF from opu oocytes successful in reaching morula stage of 32 cells but fail to reach blastocyst stage. Can any one...
20 July 2022 1,392 4 View
Hi friends, While carrying out MTT assay, I encountered with a value of 6 for one error bar of the experiment groups. So, I would like to ask what is the highest accepted error value in...
12 July 2022 8,076 2 View
I was looking for expression of ribosomal subunit L10a in pancreas and it feels like there is difference in staining. I was wondering if the translation machinery in acinar vs ductal cells different?
12 July 2022 620 0 View
Hi folks! I'm trying to stain for Tfh and B cells on PP for confocal microscopy but I don't have a good protocol. Also, is it better to use OCT or paraformaldehyde/sucrose? A working protocol...
12 July 2022 2,793 0 View
If I have to develop a Rotenone-induced Parkinson's model in the SH-SY5Y cell line, and further check the neuroprotective effect of my synthesized compound, does it really affect whether I treat...
10 July 2022 1,589 2 View
I digested 5ug of pLentiv2 (12kb) with 1uL BamHI-HF (NEB) at 37C for 2 hours in a 20uL volume with no heat inactivation required. I ran the digested vector on a gel (lane 4), and detected the...
09 July 2022 4,897 2 View
Hi all, I am planning to do FosTRAP experiment. I have Fos-creERT2 mice and I order tamoxifen (TAM) and 4-hydroxytamoxifen (4-OHT). TAM was used for this experiment in many previous papers, but...
07 July 2022 1,434 0 View
Predictability of Market returns
06 July 2022 435 0 View
What do you mean by optimum utilization of water resources? What is your approach for sustainable utilization of water resources?
06 July 2022 4,047 4 View
Hello, I have to isolate PBMCs and the centrifugation is without brake. My centrifuge is: Brai4, Jouan. Could you help me, please? Thank you.
05 July 2022 8,098 1 View
We are trying to make NOR for adult Wistar rats in our lab. The literature varies on the dimensions that should be used. I wanted to know what can work best for this. any kind of help is appreciated.
03 July 2022 4,349 2 View
Hi everyone, I recorded the LFP signal in two different conditions of the rat brain Amygdala. Under different conditions, the power spectral density(PSD) values in the delta, theta, beta and gamma...
30 June 2022 7,357 0 View
Hi everyone, I have been isolating extracellular vesicles from an osteosarcoma cell line for awhile now. I use the ultracentrifugation method (300g 10' + 16500g 20' + syringe filtering of the...
27 June 2022 459 1 View
I have these squiggles that are appearing in my GAD67 IF stain and I'm not sure why. I've used this secondary antibody (Cy5 goat anti-mouse) with another primary antibody and never had this...
27 June 2022 2,037 9 View
Planning to undertake antiviral study for project purpose. Pls suggest model and how it can be done scientifically??
25 June 2022 2,429 0 View
Hi everyone, I am currently working with X. laevis and I would like to collect blood samples. This would happened right before euthanasia on anesthetized individuals. My aim is to measure...
21 June 2022 8,659 0 View
I'm developing a lateral flow immunoassay in competitive format, and once I printed and built the complete strip I test it with different concentrations of the analyte (resuspended in acetate...
17 June 2022 1,605 0 View
I'm trying to isolate mouse bone marrow mesenchymal stem cells. My simple version protocol : 1. Whole bone marrow flushing with syringe and PBS. 2. large tissues removed with cell...
13 June 2022 6,633 3 View
Hey, Im haveing trouble detecting the mouse in a OFT, and if I do have luck detecting the animal in one frame, when I see the rest of the frames, the mouse is undetected so I try to detect it on...
13 June 2022 6,213 2 View
Hi all, I am currently planning to make the allergy model mice by injecting OVA and aluminum hydroxide (as adjuvant) dissolved in saline. However, reading papers, it seems like the amount of...
11 June 2022 3,204 2 View
Hello, I would like to perform immunofluorescent staining of IgG and C3 (immune complexes) in the kidneys of lupus mice. I was wondering, whether perfusion of the mice is required for this,...
08 June 2022 6,766 2 View
Currently we have a two-chamber organ-on-a-chip setup of three cell types (immune cells+cell type A in one chamber, cell type B in another chamber) where we want to measure immune cell migration...
08 June 2022 5,589 2 View
Is the antigen retrieval process required for frozen sections of muscle tissue, if required then how we can do it? what kind of reagent is necessary for the amplification of signals?
07 June 2022 2,521 4 View
Hello, We would like to measure the pH of bile sample from the mice. Considering the small volume of bile we can collect (5-10ul), can anyone suggest the best method to measure bile pH and...
07 June 2022 1,445 0 View