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Questions related to Mice
Hello everyone, A collaborator sent us his protocol in order to be able to use his immunohistochemistry kits. But one step contains PBS + 0.3% H2O2 + 0.1% sodium azide. Knowing the dangerousness...
24 July 2024 6,291 3 View
Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....
24 July 2024 7,263 3 View
I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...
23 July 2024 9,136 2 View
I was performing estimation of Total Soluble Sugar content in bacterial cultures by Anthrone method. Out of 9 samples, only one sample showed positive reading while others had a negative reading...
23 July 2024 9,550 4 View
Hi, I am a PhD student working at Jagiellonian University, Faculty of Chemistry. My work is related to the development of new analytical methods for the quantification of short-chain fatty acids...
23 July 2024 5,470 6 View
I'm facing issues with the tumor dissociation protocol for GBM. I use collagenase/dispase with DNase to dissociate my tumor sample. However, even after incubating it for 60mins, I do not get a...
23 July 2024 392 3 View
I have used 30 years of daily data, but the model is not able to optimize, getting just 0.57 in NSE and R2. when I reduced the data to 20 years in daily time frame I got 0.73 in NSE, R2 for both...
23 July 2024 644 0 View
Im trying to transfect A549 cells using lipfectamine 3000. im using a 96 well plate with 1microgram plasmid DNA in each well . should i remove the culture media or change it or decrease it befor...
22 July 2024 7,588 2 View
How does grey - water harvesting improve the ground water table and what would happen if we use up ground water faster than rainwater can add to it?
22 July 2024 8,415 2 View
Hello, I am a master's student currently conducting lentivirus transduction experiments in Jurkat cells. The virus is not effectively entering the Jurkat cells, so I am planning to use an...
22 July 2024 4,653 4 View
Despite not having cells in the media, I am getting purple color. I have tried many troubleshooting methods, varying media types, and even different MTTs from different companies to figure out the...
22 July 2024 9,890 1 View
I would like to know about aristolochia bracteolata research articles and reviews on different website such as Google scholar and research gate and other sites
22 July 2024 5,394 1 View
Actually, I'm having trouble implementing the plot of the flutter region for the whirl prop system with 2 DOF (Influences of structural damping and propeller—pivot point distance on whirl flutter...
22 July 2024 5,020 2 View
science teachers' professional vision:framewor,evaluation,review of related research,researchers related,research method,why professional vision
21 July 2024 6,539 1 View
I have conducted a study on the fructose, glucose, and sucrose compound content in tomato flesh extracts. The obtained peak areas aligned with the chromatogram graph. However, the concentrations...
20 July 2024 424 2 View
I am currently working on a mendelian randomization study, and I have downloaded the datasets needed from the ieu opengwas project (mrcieu.ac.uk) in .vcf format. I do not have access to an R...
20 July 2024 2,317 5 View
nursing sustainability practice questionnaire?
20 July 2024 9,925 1 View
Kindly confirm how to distinguish glia cells and neurons using cresyl violet staining. Also, using cresyl violet nissl staining how to identify the apoptotic neurons ? Does this stain stains...
19 July 2024 2,393 0 View
Hello all, I extracted RNA from my samples and performed RIP-seq. After annotating the genomic regions using R, I obtained promoters, exons, introns, and UTRs. Given that my samples consist of...
19 July 2024 1,547 2 View
I want to write article in a topic of positive role of politics in nursing, and I'm confused how can we add add questionnaire and what will be the questionnaires.
19 July 2024 9,999 3 View
What are the ways to integrate environmental and sustainability issues into science curricula: in response to environmental challenges in the Arab Gulf countries such as the Emirates, Qatar and...
19 July 2024 8,051 0 View
We have mice brains that were over-fixed due to old PFA used during perfusion. Thus, the synapses are no longer being labeled by the Synaptophysin (SY38) mAB. which works perfectly every other...
18 July 2024 7,744 3 View
How can the economic value of digestate management, utilization, and regulation, can spark interesting discussions and explorations? so one can list down the details and we can discuss in length...
18 July 2024 3,229 4 View
How use this chamber and standarize the data
18 July 2024 1,823 2 View