I have prepared a liquid broth containing Yeast (S. Cerevisiae) that i need to add from it to fermentation media of lignocellulosic hydrolysate.
I need to know based on what parameters do we add milliliters of yeast to fermentation broth?
Hello
If you're working with a lignocellulosic hydrolysate and S. cerevisiae I imagine that you are about to work with an anaerobic or a micro-aerated fermentation.
In this case, you desire to produce a high amount of ethanol. So, preparing the inocula for a fermentation experiment is one of the crucial steps. If you add a small amount of yeast cells, the ethanol yield, the substrate consumption, and the product formation rates would be low.
Usually, work with an inoculum interval of 5 to 10% of the total work volume is useful if you are only interested in obtaining the final product and your propagation medium has the same chemical composition as the fermentation medium.
Contrarily, if you are interested in a kinetic evaluation of the whole fermentation process, you should control the inoculum size by measuring the initial biomass of the yeast strain.
I recommend these articles for further reading:
Article The Influence of Inoculum Level on Fermentation and Flavour ...
Article Inoculum Size and Age Studies on Single and Mixed Strain Fer...
https://doi.org/10.5897/AJB2013.13179I hope I've helped.