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Questions related to Mice
I am using a construct that does not have a TEV cleavage site between my protein and the 6xHIS tag, and I was wondering if I could use Q5 site directed mutagenesis kit to insert it or would I need...
17 April 2022 9,033 4 View
I am trying to undergo a cell viability assay with trypan blue staining. I originally got cell suspension by pipetting and observe cells after staining on the hemocytometer. But I found out my...
16 April 2022 9,985 4 View
Hello everyone. Now, I am isolating colonic fibroblasts from C57BL/6 mice, I have tried digestion with 0.05g/ml collagenase type 1, but very few fibroblasts are isolated. Can any research...
13 April 2022 7,684 2 View
We are trying to measure the contractile force generated by primary aortic vascular smooth muscle cells (mouse), and we need a chemical stimulant to initiate a temporary contraction of the cells...
12 April 2022 7,863 2 View
I want to extract DNA from mice fecal samples for the pathogen detection. for that, I want to ensure there is a DNA in that sample by using housekeeping gene's primers. if you have any ideas...
11 April 2022 2,988 2 View
If the tissue is old tissue, can these soluble factors be measurable?
09 April 2022 9,003 0 View
Most of the open literature explains that the QuEChERS followed by dSPEmethod may be suitable, but from my practical experience, it was found that it is not suitable for such types (spices) highly...
08 April 2022 6,623 8 View
I have read different protocolos both fast and slow instillation methods and using various volumes of bacterial suspension. I would need to know a standard protocol for intranasal instillation...
07 April 2022 3,461 3 View
Hello. So I had seeded some 48-well plates with 70,000 cells/well (human osteoprogenitor) on a calcium phosphate substrate scaffold. After 24 hours I wanted to study the overall attachment so...
06 April 2022 3,605 4 View
Dear all, I'm searching a validated protocol to obtain lymphocytes from whole blood to mantain them in culture and obtain from them iPSC. If someone can give me a protocol starting from whole...
06 April 2022 3,253 2 View
Hello every one, I wants to separate, purify and culture the mice uterus epithelial cells and there are various protocol available, kindly if any one worked in this area suggest me the standard...
05 April 2022 8,388 3 View
Hello, I would like to detect ROS using DHE in tumor sample in situ. I have thought of the following scenario: - to inject 20 mg/kg DHE to mice ip and after 1 hour to sacrifice the mice, take out...
04 April 2022 3,283 3 View
Hello, Does anybody know how to isolate CD34+ cells from mouse bone marrow? Is there any kit available? Thank you. KIYO
02 April 2022 2,016 3 View
Hello, has anyone tried any delipidation method for brain slice culture? I am using hippocampal slice cultures and need to remove lipids from the 2 weeks cultured tissue for imaging purposes. Thanks
31 March 2022 7,166 0 View
I want to try using DAPI on frozen sections. Is the protocol different from paraffin sections? Should i use triton?
31 March 2022 493 3 View
Ideally recorders that can be left out for weeks at a time, with frogs from tropical forests the target animals.
31 March 2022 3,546 10 View
I've read that there are several methods for the administration of exosomes to mouse models (e.g. intravenous/tail-vein, retro-orbital, intraperitoneal, intranasal, and subcutaneous) and I was...
30 March 2022 7,464 3 View
Dear all, I am just trying to do an overall stimulation of PBMCs. Anti CD28/CD3 to stimulate T cells, but how can I stimulate the innate response? Some TLR agonist should be fine? TLR agonist can...
30 March 2022 9,922 4 View
Is there a technique for DMEM as well, as most articles advise William's E Media? Can someone share the protocol for isolating and primary culture of mouse hepatocytes in DMEM or suggest articles...
28 March 2022 3,503 1 View
Hello! For a PBMCs culture I prepared a media with RPMI-1640, NaOH, B-ME, antibiotics and Fetal bovine serum. After extraction of the PBMCs from whole blood, I washed them twice with alsever...
28 March 2022 6,839 4 View
Dear all, I need to differentiate osteoblasts and osteoclasts from mouse bone marrow and I am following this detailed protocol: Isolation, Culture, and Differentiation of Bone Marrow Strom......
25 March 2022 1,833 3 View
I would like to know how much volume of a blocking solution and the volume of antibodies solution works best for a cell suspension. We resuspend the cells in 500 µL of blocking solution and then...
25 March 2022 806 1 View
If there is a G4 PAMAM dendrimer solution (in methanol), what are the suitable temperature, vacuum level, conditions, and time period for 5ml methanol removal from the solution using a rotary...
24 March 2022 3,761 3 View
Hello everyone, I have a question regarding a statistic test. I will give you the background first. I am testing different characteristics of microglial cells. I am testing the difference between...
23 March 2022 4,148 3 View