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Questions related to Mice
Hi everybody, i need to pick your brains: i work with an adhesive fish cell line which forms a konfluent monolayer. I want to test an adsorbant substance, therefore i want to saturate the cell...
01 July 2024 9,332 0 View
Respected researcher, I want to know that is there any way or one can help in generating a 2D/3D image using the energy count spectrum of from backscatter methods. How can I design such an...
30 June 2024 10,049 1 View
I would like to find a short course focused on the development of monoclonal antibodies covering the entire process, from the selection of antigen and carrier, to the purification of the final...
28 June 2024 5,638 1 View
The Evolution and Application of Spinal Anesthesia: Techniqu... What are the key contraindications to neuraxial anesthesia, and how are they managed?
28 June 2024 3,137 1 View
The Evolution and Application of Spinal Anesthesia: Techniqu... How do you educate patients about the potential risks and benefits of neuraxial anesthesia, and what postoperative instructions do...
28 June 2024 693 1 View
The Evolution and Application of Spinal Anesthesia: Techniqu... What are some of the most common complications associated with neuraxial anesthesia, and how are they prevented or managed?
28 June 2024 8,686 1 View
Overview of Normal Saline in Fluid Therapy: Mechanisms, and ... How might future research impact the currentpractices of fluid therapy in critically ill patients?
28 June 2024 4,485 1 View
The question explores how public administration can leverage community advocacy to tackle health disparities. It seeks to understand the strategies and approaches that public administrators can...
28 June 2024 1,317 3 View
I would like to know whether it is possible for humans to create or manufacture other humans who can do everything a human can also do. I mean is it possible to create a human being without...
28 June 2024 4,528 3 View
I am trying to quantify the release of Nitric oxide to test the Immunogenicity of my antigen on U937 cells (Monocytes). Based on the literature, I am supposed to convert 1x105 cells/ml to...
28 June 2024 3,173 2 View
Dear All, I found a paper related to my work. I will do the same thing except I will change the reagent they use. For example, instead of using reagent X, I will use reagent Y. They use gold...
26 June 2024 5,820 0 View
thanks
26 June 2024 4,700 0 View
I am trying to grow microglia culture for 10 days and after day 2/3 there are certain flat non-microglial contaminating cells observed. They are rapidly growing and some are even growing on top or...
25 June 2024 5,341 2 View
How does the overuse of chemical fertilizers and pesticides affect the environment? How do fertilizers and pesticides affect soil health and biodiversity?
23 June 2024 6,183 4 View
Good evening, I´m establishing a protocol for detecting ROS using DHE staining in Flow Cytometry using TK6 cells. They look visibly pink after staining them with 10 micro Molar DHE. Is that...
20 June 2024 7,366 0 View
I would like to know whether a PhD degree is mandatory or not to become an assistant professor in India. Will it be an added advantage to improve the overall academic performance indicator (API)...
20 June 2024 9,374 1 View
When I applied the cell cycle algorithm to these two samples in FlowJo, I encountered some concerns regarding the accuracy of the fit. The images include both a control and an apoptosis-inducing...
19 June 2024 2,011 0 View
(Transfection with two plasmids has worked for this cell line. pRL-TK(Renilla) is not getting transfected).
17 June 2024 6,713 2 View
Comprehensive Overview of Myocardial Infarction: Etiology, E... What factors influence the prognosis of patients who have had a myocardial infarction?
16 June 2024 8,593 1 View
Comprehensive Review of Thrombolytic Therapy: Mechanisms, Ap... How should thrombolytics be administered, and what are the key considerations in their administration?
16 June 2024 2,823 1 View
Is there scientific evidence that proves that the presence of N-acyl homoserine lactone can account for the bacterial biofilm population?..
14 June 2024 3,803 2 View
Hey all! Thank you for taking the time to read and help if possible! For some organotypic culture experiments I need to differentiate IPSC into vascular smooth muscle cells Does anybody have a...
12 June 2024 6,846 0 View
Is it possible to do FACS on snap frozen cells ? They were isolated and directly dropped in liquid N2. They were not frozen with DMSO or FBS.
12 June 2024 8,934 0 View
if MCF7 is injected into mice, will cancer develop?
07 June 2024 9,442 2 View